FastQCFastQC Report
Wed 29 Oct 2014
16hr_13_ATTCAGAA-CCTATCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_13_ATTCAGAA-CCTATCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3315972
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA178850.5393591984492029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG7900.013.9569082
ATATGGA15500.013.3626481
ATATCTC11400.011.65154651
TATGGAT20350.010.6151712
ATCTCGA17750.010.5272791
TCTCGAG18000.010.5009122
GGATCCG23800.010.4941344
TCGAGGG19700.09.7085254
GATCCGG32050.09.4126781
CTCGAGG20650.09.15333753
CGAGGGC22200.08.6152235
ATCCGGC35800.08.4225192
TGGATCC27000.08.3340573
TCGGCGC3602.6930316E-47.5006513
GAGGGCG25850.07.4854666
CGGCGCG45250.07.35942175
AGGGCGC26450.07.2303797
TCCGGCG43600.07.17378763
GGGCGCG28150.06.87365778
GCCGTCG49800.06.77657211
Produced by FastQC (version 0.11.2)