FastQCFastQC Report
Wed 29 Oct 2014
16hr_12_ATTCAGAA-ATAGAGGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_12_ATTCAGAA-ATAGAGGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4929987
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270062.5761934057838287No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT63630.12906727745935234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA26200.021.9089981
TGCGCGC2700.021.6694453
ATCTCGA34200.017.8372061
TATGGAT34800.016.6833442
TATCTCG16850.016.0259572
TCGCGCC1552.067045E-715.969793
TCTCGAG39500.015.7806652
ATATCTC20600.013.3314151
TCGAGGG47500.013.1699764
GTCGCGC1555.8098078E-513.0663242
CTCGAGG47750.012.8655233
TGGATCC50550.011.8411614
CGAGGGC53650.011.8280525
GGATCCG51500.011.5790385
ATGGATC52100.011.3594233
CTGCGCG2901.684457E-710.8635732
TGGGCGC1954.914657E-410.3859483
GAGGGCG63200.010.074516
ATAAAAA20600.09.8314582
AATAAAA20050.09.5430721
Produced by FastQC (version 0.11.2)