FastQCFastQC Report
Wed 29 Oct 2014
16hr_12_ATTCAGAA-ATAGAGGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_12_ATTCAGAA-ATAGAGGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2434497
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380481.5628690444062983No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT29330.12047663233924708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG8300.019.7918662
ATATGGA12600.019.4769551
GTCGCGC706.9801055E-419.2883552
TGCGCGC1354.271351E-818.3358443
ATCTCGA15100.016.2522951
TATGGAT15800.015.9515522
ATATCTC10750.014.8701371
TCTCGAG18650.013.2725962
CTGCGCG1600.001107397811.2515412
GGATCCG24450.011.1364945
TCGAGGG23000.010.9580224
CTCGAGG22550.010.8773213
TGGATCC24550.010.8161454
ATGGATC25850.010.5333573
CGAGGGC25850.010.0110425
GCACACG3502.2082695E-68.99993830
TATACGA3603.2355892E-68.74940113
GATCCGG31850.08.6191736
GAGGGCG29600.08.5901746
ATCCGGC37150.08.1816341
Produced by FastQC (version 0.11.2)