FastQCFastQC Report
Wed 29 Oct 2014
16hr_11_ATTCAGAA-TATAGCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_11_ATTCAGAA-TATAGCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2675006
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA454531.699173758862597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA13000.023.0299741
TATCTCG7750.018.5830632
TATGGAT16150.017.9744742
ATCTCGA17050.015.8431681
TGGGCGC1004.16609E-415.7517533
TCTCGAG17850.015.5062182
ATATCTC9600.015.2414861
TCGAGGG20750.013.8810634
CTCGAGG21100.013.0109273
TGGCGCG1607.821454E-512.657663
GTGGGCG1250.002250671412.6016382
TGCGCGC1454.8477063E-412.4151753
CGAGGGC24200.012.1811085
TGGATCC25750.011.7100414
GGATCCG25400.011.5170315
ATGGATC28100.010.8108123
GAGGGCG27850.010.582886
ATAAAAA7250.010.2427112
AGGGCGC28550.010.1657967
GATCCGG30050.09.8829656
Produced by FastQC (version 0.11.2)