FastQCFastQC Report
Wed 29 Oct 2014
16hr_11_ATTCAGAA-TATAGCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_11_ATTCAGAA-TATAGCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3367547
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340301.01052784118529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA16750.020.296851
TATCTCG9950.016.9623242
TATGGAT21800.016.1033132
TGCGCGC1402.2293594E-514.4664383
GTCGCGC1108.5847336E-414.3203132
ATATCTC11600.013.9746521
ATCTCGA19400.013.2302981
TCTCGAG21200.012.7377442
TGGATCC32000.011.5324534
TCGAGGG25200.011.4297644
GGATCCG32250.011.02440555
TCGCGCC1450.006821627710.8636863
CTCGAGG24700.010.6594813
ATGGATC35400.010.4252813
CGAGGGC28850.09.9837095
GATCCGG39350.09.091226
GAGGGCG33900.08.4953266
AGGGCGC34300.08.3960067
ATCCGGC43450.08.2848967
GGGCGCG36050.07.9260238
Produced by FastQC (version 0.11.2)