FastQCFastQC Report
Wed 29 Oct 2014
16hr_10_GAGATTCC-GGCTCTGA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_10_GAGATTCC-GGCTCTGA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441652
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1651796.765050875390924No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT31630.1295434402609381No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30040.12303145575208915No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT26820.10984366322473472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA14100.026.5086271
ATATCTC10250.019.5507951
TATCTCG10200.019.416732
TATGGAT19850.019.1610722
TGCGCGC1102.25258E-618.4138013
ATAAAAA12900.017.0973972
AATAAAA13600.015.8939061
ATCTCGA17750.015.3491831
TCGTATG2651.8189894E-1215.28223345
CTGCGCG1056.028592E-415.0038392
ACGTCTG2850.014.99922915
TGGATCC28800.014.3005324
ATTCGGC2052.1605956E-814.2786691
CACACGT2905.456968E-1213.964812
GGATCCG29200.013.8734115
ATGGATC28350.013.4955153
CTCGTAT3303.6379788E-1212.95387944
TCTCGAG22500.012.4031722
CTCGAGG25700.011.2090933
TCGAGGG26350.011.1034094
Produced by FastQC (version 0.11.2)