FastQCFastQC Report
Wed 29 Oct 2014
16hr_10_GAGATTCC-GGCTCTGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_10_GAGATTCC-GGCTCTGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4293430
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750844.077951661026265No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT55960.13033868026263384No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53280.12409658478186437No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47730.11116985720042018No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT43530.10138746876040834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA26050.025.1459731
TATCTCG18650.020.0283642
TATGGAT34450.019.5951352
ATATCTC21300.017.754681
ACGTCTG5050.016.92964215
GCACACG5650.015.92821511
CACACGT5800.015.12837112
TCGTATG6100.014.75472845
CTCGTAT6400.014.063144
ATCTCGA30550.013.9999221
ATAAAAA21700.013.6876982
AATAAAA21950.013.4344521
TGGATCC52550.013.3594324
ATGGATC51950.013.2108423
GGATCCG54450.012.9759125
TGCGCGC3001.7389539E-912.0009313
ACACGTC7550.011.91978813
TCTCGAG37800.011.4889882
CACGTCT8300.010.842714
AGTCACG8650.010.40446228
Produced by FastQC (version 0.11.2)