FastQCFastQC Report
Fri 19 Dec 2014
wt_PNK_GATCAG_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamewt_PNK_GATCAG_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8600132
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1272831.4800121672551074Illumina Single End PCR Primer 1 (100% over 51bp)
CCAAGAACAAGTTTAACCGCTCATTACTCCAAACGGATTTTTTTGCCAGAT128180.14904422397237624No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC136450.043.10434344
GTCGCCG138000.042.6691145
TGGTCGC139050.042.18509343
GTGGTCG139900.042.13786742
GGTGGTC145600.040.5191541
CGGTGGT151150.039.2395240
GCACACG81500.038.4843418
CACACGT75000.036.5401219
AGCACAC91200.035.08194417
GAGCACA94900.035.01814716
AGAGCAC97400.034.35032715
ACACGTC50650.033.6281420
CTCGGTG184050.032.5791938
TCTCGGT186800.032.15979437
ACCGCTC24500.032.1425916
TCGGTGG188850.031.95403739
CACGTAG30950.031.91466721
ATCTCGG194900.031.2395636
CCGCTCA25950.030.43327117
CGCTCAT26550.029.74551418
Produced by FastQC (version 0.11.2)