FastQCFastQC Report
Fri 19 Dec 2014
wt_PNK_GATCAG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamewt_PNK_GATCAG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8600132
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1149501.3366073916074777TruSeq Adapter, Index 9 (100% over 50bp)
GGCAAAAAAATCCGTTTGGAGTAATGAGCGGTTAAACTTGTTCTTGGAGAT128160.14902096851536697No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC91770.10670766448701019TruSeq Adapter, Index 9 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC155200.038.09670345
CTCGTAT145600.037.00819843
TCGTATG161400.036.61932444
TCTCGTA147750.036.3630842
ATCTCGT149250.035.92224541
GATCTCG153250.034.69101740
GAGCGTC26450.029.85624716
AGCGTCG23600.029.17179917
AGAGCGT30400.029.01123815
GAGCGGT24800.028.57673626
AGATCTC189100.028.185639
TGAGCGG25000.028.07814225
CAGATCT192550.027.58711238
GCGGTTA26250.027.16963228
ACGACGC20550.026.38511516
ATGAGCG27200.026.13797624
AAGAGCG35700.026.09071214
GATCAGA224650.026.04880135
TCAGATC205450.025.887837
ATCAGAT226150.025.71684836
Produced by FastQC (version 0.11.2)