FastQCFastQC Report
Fri 19 Dec 2014
wt_ATCACG_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamewt_ATCACG_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22023995
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG109200.037.17250418
AGCACAC136350.034.70586817
AGAGCAC166100.034.02007715
GAGCACA164950.034.01122316
CACACGT65850.031.74445319
ACACGAG61850.031.24932520
CACACGA68150.028.8901719
CACGAGA69300.028.1499121
GGTCGCC23450.026.28703944
ACACGTC43700.025.48654620
AAGAGCA278400.023.49816914
CACGTAG40550.023.41600221
GTCGCCG27200.023.07646445
ACGAGAT85150.022.72536522
ACACGTA45350.021.43352720
CGAGATC90650.021.12315823
CGGGGAG21750.021.0025651
CGGGGTG19500.020.0793721
CCGGGTC12500.019.6223961
CAGATCG162200.019.4090546
Produced by FastQC (version 0.11.2)