FastQCFastQC Report
Fri 19 Dec 2014
wt_ATCACG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamewt_ATCACG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22023995
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGTGCCCCGGGTAACCCAGTTCCTCACTATTTTTTACTGCGGAAGCGGA253490.11509719285715421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCCCC58200.019.6043594
TGCCCCG58450.019.3658835
CCCCGGG58950.019.0822537
CCCGGGT62350.018.0055968
CAGGTGC72650.016.057961
CCGGGTA72650.015.8554219
GGTGCCC75600.015.212043
GCCCCGG77600.014.8162776
CGTGTGC174750.014.6792131
ATCACGA121450.014.31993945
CATCACG123200.014.17131644
AGGTGCC82450.013.8117352
CGGGTAA88000.013.39691610
GTGTGCT198950.012.8505812
GGGTAAC90400.012.79236711
GTAACCC93750.012.23928713
GGTAACC97350.011.71734512
ACGTGTG123300.011.7082271
TGTGCTC225950.011.3050343
CTCGTAT48700.011.2725243
Produced by FastQC (version 0.11.2)