FastQCFastQC Report
Fri 19 Dec 2014
rad27d_CGATGT_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamerad27d_CGATGT_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14024219
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG992730.7078682955535706Illumina Single End PCR Primer 1 (100% over 51bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC355120.25321909191520753Illumina Single End PCR Primer 1 (100% over 40bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG349170.248976431414826Illumina Single End PCR Primer 1 (100% over 41bp)
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT197450.14079215391602198Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTTCCCTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT175170.12490535123560179Illumina Single End PCR Primer 1 (100% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC112650.040.76120444
GTGGTCG115600.039.7399142
TGGTCGC114700.039.678543
GTCGCCG116900.039.3562745
ACACTCT173050.037.070941
GGTGGTC124650.036.89141541
GCACACG94800.036.5285218
CGGTGGT134700.034.72375540
CACTCTT190450.034.2886772
AGCACAC106950.034.251917
CTCGGTG141050.033.71732338
GAGCACA123200.033.2244516
AGAGCAC124000.033.2098415
CACACGT69400.032.71398519
TCGGTGG150750.031.6973639
TCTCGGT152200.031.21989437
CTCTTTC239250.030.1073574
ACTCTTT236150.029.893153
CTTTCCC149600.029.8870816
CCCTAAG48550.029.65764810
Produced by FastQC (version 0.11.2)