FastQCFastQC Report
Fri 19 Dec 2014
rad27d_CGATGT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamerad27d_CGATGT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14024219
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC967260.689706856403198TruSeq Adapter, Index 2 (100% over 50bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT348720.24865555793160388TruSeq Adapter, Index 2 (100% over 39bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA343110.24465533517410132TruSeq Adapter, Index 2 (100% over 40bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG199010.1419045153245254TruSeq Adapter, Index 2 (100% over 38bp)
TAGGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA172410.1229373272051727TruSeq Adapter, Index 2 (100% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC129800.036.41742745
TATCTCG124450.036.15588840
CTCGTAT130600.035.0745843
TCGTATG139700.034.06213444
TCTCGTA134100.033.87379542
ATCTCGT134500.033.82311641
GTATCTC155100.029.112539
ATGTATC166000.027.14668737
TGTATCT171900.026.25421338
GATGTAT212350.022.02653336
TGTAGGG61500.020.212641
GTAGGGA62850.019.9081612
CGATGTA308650.016.53235245
CAGACGT64600.016.0245913
TCAGACG67950.015.2014442
GACGTGT107700.014.9502851
GTCGTGT31550.014.4083292
TAGGGAA113500.014.1466231
CGTCGTG33600.013.9406541
AGACGTG74000.013.9276684
Produced by FastQC (version 0.11.2)