##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	lane2_Undetermined_L002_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3433129
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.415340349867424	33.0	15.0	33.0	6.0	33.0
2	26.11086533596611	33.0	15.0	33.0	6.0	33.0
3	25.98674765789459	33.0	15.0	33.0	6.0	33.0
4	28.795333644613994	37.0	15.0	37.0	6.0	37.0
5	28.865246543313695	37.0	15.0	37.0	6.0	37.0
6	28.788356044879176	37.0	15.0	37.0	6.0	37.0
7	29.257604942896116	37.0	15.0	37.0	6.0	37.0
8	28.861953628890728	37.0	15.0	37.0	6.0	37.0
9	29.374415292871312	37.0	22.0	37.0	6.0	37.0
10	28.84078168924034	37.0	15.0	37.0	6.0	37.0
11	28.861244654657604	37.0	15.0	37.0	6.0	37.0
12	28.818085192837206	37.0	15.0	37.0	6.0	37.0
13	28.754537333144196	37.0	15.0	37.0	6.0	37.0
14	30.700143222116036	40.0	15.0	40.0	6.0	40.0
15	30.830032894190694	40.0	15.0	40.0	6.0	40.0
16	30.259050562912144	40.0	15.0	40.0	6.0	40.0
17	30.676956502362714	40.0	15.0	40.0	6.0	40.0
18	30.277323980543695	37.0	15.0	40.0	6.0	40.0
19	30.234058492995747	40.0	15.0	40.0	6.0	40.0
20	30.657148624476388	40.0	15.0	40.0	6.0	40.0
21	30.3423847458106	40.0	15.0	40.0	6.0	40.0
22	30.752728196347995	40.0	15.0	40.0	6.0	40.0
23	30.309383072992595	40.0	15.0	40.0	6.0	40.0
24	30.102354149814936	40.0	15.0	40.0	6.0	40.0
25	29.771203470653155	37.0	15.0	40.0	6.0	40.0
26	29.893265298216292	37.0	15.0	40.0	6.0	40.0
27	29.81457119729553	37.0	15.0	40.0	6.0	40.0
28	29.839883674630343	37.0	15.0	40.0	6.0	40.0
29	30.12119527113604	37.0	15.0	40.0	6.0	40.0
30	30.13688387473934	37.0	15.0	40.0	6.0	40.0
31	29.90891923956251	37.0	15.0	40.0	6.0	40.0
32	29.797670288532707	37.0	15.0	40.0	6.0	40.0
33	29.801329341251087	37.0	15.0	40.0	6.0	40.0
34	29.70509905104061	37.0	15.0	40.0	6.0	40.0
35	29.707894168847137	37.0	15.0	40.0	6.0	40.0
36	29.627249660586596	37.0	15.0	40.0	6.0	40.0
37	29.647754278968254	37.0	15.0	40.0	6.0	40.0
38	29.621267071525715	37.0	15.0	40.0	6.0	40.0
39	29.638104481363793	37.0	15.0	40.0	6.0	40.0
40	29.47345759509765	37.0	15.0	40.0	6.0	40.0
41	29.4847187507373	37.0	15.0	40.0	6.0	40.0
42	29.349870628222824	37.0	15.0	40.0	6.0	40.0
43	29.187008702556763	37.0	15.0	40.0	6.0	40.0
44	29.20199998310579	37.0	15.0	40.0	6.0	40.0
45	29.012445206690458	37.0	15.0	40.0	6.0	40.0
46	28.85975068224934	37.0	15.0	40.0	6.0	40.0
47	29.047225431960175	37.0	15.0	40.0	6.0	40.0
48	28.920386621067838	37.0	15.0	40.0	6.0	40.0
49	28.813013725962527	37.0	15.0	40.0	6.0	40.0
50	28.687055161632436	37.0	15.0	40.0	6.0	40.0
51	28.08187953321882	37.0	15.0	40.0	6.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.10275493151394244
2201	2	0.09754835611854773
2201	3	0.05144980906204211
2201	4	0.13182836575996504
2201	5	0.029787055281438768
2201	6	0.04192401431225434
2201	7	-0.0372070710832233
2201	8	0.02248968143880603
2201	9	-0.08992238828984966
2201	10	0.08102606239798504
2201	11	0.06599476198534404
2201	12	0.033225149272890775
2201	13	0.09200492448691477
2201	14	0.23249673805652904
2201	15	-0.009934011337072945
2201	16	0.11581584239913312
2201	17	-0.03219856726644821
2201	18	0.11195006425079157
2201	19	0.18287824689793197
2201	20	-0.004267085245540869
2201	21	1.8219449413692246E-4
2201	22	0.03661938295480027
2201	23	0.06761378711368593
2201	24	0.17742171734360568
2201	25	0.05009366547482941
2201	26	0.21337330795877918
2201	27	0.2370782744650768
2201	28	0.14861013032858494
2201	29	0.1250040732761093
2201	30	0.04917739814777278
2201	31	0.10276951622222441
2201	32	0.09687483211854442
2201	33	0.06445538455309574
2201	34	-0.030053057763499424
2201	35	0.029260358057289437
2201	36	0.21666629769518408
2201	37	0.1411455764384364
2201	38	-0.011880589486128912
2201	39	0.014136342480117747
2201	40	0.20862650564731666
2201	41	0.1923803109023332
2201	42	0.2242410684104037
2201	43	0.18257490975146595
2201	44	0.16401094881630485
2201	45	0.29039996626220344
2201	46	0.18035469542967064
2201	47	0.2456700489134036
2201	48	0.31402393199275735
2201	49	0.18095848070094434
2201	50	0.1319831294145608
2201	51	0.19722902235649187
1101	1	-0.13243314792847727
1101	2	-0.2013853504976275
1101	3	-0.14886432566259344
1101	4	-0.1790717150247687
1101	5	-0.19487713119026395
1101	6	-0.2405818962806734
1101	7	-0.2313321695328341
1101	8	-0.17163577862555002
1101	9	-0.3512054914071854
1101	10	-0.16041325105473447
1101	11	-0.11525847898676389
1101	12	-0.14710922864489007
1101	13	-0.22151469347434372
1101	14	-0.17850336536528744
1101	15	-0.21537982083697926
1101	16	-0.20127689080802114
1101	17	-0.21100132595297794
1101	18	-0.13098904633605812
1101	19	-0.20659402701510743
1101	20	-0.2081534311792197
1101	21	-0.20951457708178722
1101	22	-0.3073259568075599
1101	23	-0.29355273141147364
1101	24	-0.2544638070013825
1101	25	-0.13100064581905002
1101	26	-0.10813347557212794
1101	27	-0.11251783803182391
1101	28	-0.14591513099230724
1101	29	-0.0742532423708866
1101	30	-0.17344890845668814
1101	31	-0.23220398103348217
1101	32	-0.09480303857815287
1101	33	-0.06433949093210245
1101	34	-0.0883334205643358
1101	35	-0.0868837873639059
1101	36	0.016800746055512406
1101	37	-0.08657063843882895
1101	38	0.07787996369763306
1101	39	-0.11803276014397568
1101	40	-0.03977569962401262
1101	41	0.0037322293046919697
1101	42	0.01129591162510124
1101	43	0.030465647134544582
1101	44	0.05928533123257296
1101	45	0.010222673787477987
1101	46	0.0659594996984616
1101	47	-0.07780590768414797
1101	48	0.0020502170449780976
1101	49	0.056495207378780776
1101	50	0.06053058809465384
1101	51	-0.07180710078634434
2202	1	-0.14467679420679147
2202	2	-0.24482518382830065
2202	3	-0.23793270431069047
2202	4	-0.25139144033649785
2202	5	-0.3274361241792896
2202	6	-0.2445917884887301
2202	7	-0.4194248633729458
2202	8	-0.4160892564101175
2202	9	-0.41010528382719613
2202	10	-0.34055602640509264
2202	11	-0.2926048937549588
2202	12	-0.37840374084872863
2202	13	-0.47767226930325535
2202	14	-0.4039170330237525
2202	15	-0.4668727741310619
2202	16	-0.3580397191085112
2202	17	-0.4595454915596058
2202	18	-0.3623177348323914
2202	19	-0.4595653046367403
2202	20	-0.5056211907025485
2202	21	-0.4246465194302047
2202	22	-0.46547395486711807
2202	23	-0.3553666728165794
2202	24	-0.3513591031160459
2202	25	-0.25989886604552837
2202	26	-0.336065301225112
2202	27	-0.4883438071377988
2202	28	-0.36575003385680915
2202	29	-0.37796765467737714
2202	30	-0.3032151981051818
2202	31	-0.30393035041824845
2202	32	-0.36545287576784347
2202	33	-0.4358935916247937
2202	34	-0.3789759264558583
2202	35	-0.47389574528292755
2202	36	-0.4278786399650798
2202	37	-0.4055503352829213
2202	38	-0.46990652233212415
2202	39	-0.4070568315198777
2202	40	-0.46050439871373783
2202	41	-0.3344484064836486
2202	42	-0.21362079154391722
2202	43	-0.39918953803538315
2202	44	-0.31768115077842296
2202	45	-0.28740566806961
2202	46	-0.4129634384369858
2202	47	-0.35609984229602887
2202	48	-0.3658181827899014
2202	49	-0.3451014938733401
2202	50	-0.41864974418466616
2202	51	-0.32767995825228624
1102	1	-0.48164212205288237
1102	2	-0.5329594029636446
1102	3	-0.501948649060207
1102	4	-0.5848622941317743
1102	5	-0.5857431029053934
1102	6	-0.45948778455972317
1102	7	-0.7441450998339896
1102	8	-0.5685998142452782
1102	9	-0.6335055510090619
1102	10	-0.5844655370533971
1102	11	-0.5426499919546721
1102	12	-0.5791075312515623
1102	13	-0.6035818606309284
1102	14	-0.7397282542704673
1102	15	-0.6432893726184403
1102	16	-0.6387132508321471
1102	17	-0.5751906308039665
1102	18	-0.6514070253513538
1102	19	-0.6514738299001515
1102	20	-0.6705790474825726
1102	21	-0.7213694096941268
1102	22	-0.7273475882624254
1102	23	-0.614622730720864
1102	24	-0.648079369342291
1102	25	-0.5885850417142748
1102	26	-0.6791060204150945
1102	27	-0.6404232003349613
1102	28	-0.6267800978872451
1102	29	-0.6702734670774824
1102	30	-0.6671919389485623
1102	31	-0.6008444429496507
1102	32	-0.6649912017962976
1102	33	-0.6578613300607152
1102	34	-0.7071658456864114
1102	35	-0.5996365757399396
1102	36	-0.6387936967324137
1102	37	-0.6524375020546174
1102	38	-0.7819985970725476
1102	39	-0.674854523698329
1102	40	-0.7218068143558227
1102	41	-0.6967558285546254
1102	42	-0.6919059790079132
1102	43	-0.698007389481166
1102	44	-0.7042746681520988
1102	45	-0.7249618743691038
1102	46	-0.5872430128102408
1102	47	-0.7351492121940879
1102	48	-0.7820307799723132
1102	49	-0.7173926743260601
1102	50	-0.6200096615000632
1102	51	-0.5659098372684852
2203	1	-0.7821495148261164
2203	2	-0.885422590414791
2203	3	-0.9369355730302722
2203	4	-1.0721529687781555
2203	5	-1.137195269289478
2203	6	-1.020829385969261
2203	7	-1.1166420347358077
2203	8	-1.026095077275361
2203	9	-1.1795216615046513
2203	10	-1.0754971446558734
2203	11	-1.1073165144393151
2203	12	-1.1117423199129526
2203	13	-1.1094623465292628
2203	14	-1.1035163982271143
2203	15	-1.1471064809225133
2203	16	-1.1734909878485702
2203	17	-1.1962336278019805
2203	18	-1.1384332268661517
2203	19	-1.1534518792945505
2203	20	-1.154309410371006
2203	21	-1.240355081790252
2203	22	-1.3427435708985769
2203	23	-1.1714753011590737
2203	24	-1.161962178883993
2203	25	-1.1451151955633811
2203	26	-1.1306007032724672
2203	27	-1.2177008764553925
2203	28	-1.1628576136851407
2203	29	-1.144560817909987
2203	30	-1.1834921347753031
2203	31	-1.1257731946408995
2203	32	-1.0793749823522454
2203	33	-1.1220763364566402
2203	34	-1.1807070176500467
2203	35	-1.1560016091884435
2203	36	-1.2205098416988278
2203	37	-1.1451921340250841
2203	38	-1.1880862031515171
2203	39	-1.2328571056883213
2203	40	-1.1545163020987523
2203	41	-1.099362619823097
2203	42	-1.166977079690021
2203	43	-1.161170186976534
2203	44	-1.3072652148799904
2203	45	-1.201624316453323
2203	46	-1.2236436833304545
2203	47	-1.2209042744962204
2203	48	-1.1282131180683272
2203	49	-1.1680990309078858
2203	50	-1.2663647227688095
2203	51	-1.073806238795239
1103	1	-0.3938618756967287
1103	2	-0.4504282499459116
1103	3	-0.39896468362331916
1103	4	-0.5201959833796366
1103	5	-0.5164860760965055
1103	6	-0.5309994749954718
1103	7	-0.5369587592005338
1103	8	-0.547323424652582
1103	9	-0.5720352041474221
1103	10	-0.49455174136060975
1103	11	-0.541175304356873
1103	12	-0.419135901062905
1103	13	-0.4323257697932732
1103	14	-0.5626024804712664
1103	15	-0.4935896876124879
1103	16	-0.4980409405625288
1103	17	-0.48732415107317095
1103	18	-0.44653687440419176
1103	19	-0.46826714981725104
1103	20	-0.5611232188331314
1103	21	-0.4880674368876754
1103	22	-0.3757140471615621
1103	23	-0.4778813058548437
1103	24	-0.5054818263152292
1103	25	-0.5538310539051565
1103	26	-0.4550101870373524
1103	27	-0.5023146373608611
1103	28	-0.516638277897961
1103	29	-0.5578890948903634
1103	30	-0.5990249407517112
1103	31	-0.4376114471537704
1103	32	-0.528073434523705
1103	33	-0.5396721921716114
1103	34	-0.48174550643075165
1103	35	-0.46672090907359376
1103	36	-0.4170905972723915
1103	37	-0.3819224368902816
1103	38	-0.5540411556652209
1103	39	-0.5125536234800308
1103	40	-0.5096421919368908
1103	41	-0.5332084200613139
1103	42	-0.5595876480209654
1103	43	-0.49636420768280587
1103	44	-0.5086402085204611
1103	45	-0.46808088708416307
1103	46	-0.4240671829593481
1103	47	-0.48453952285708723
1103	48	-0.4771875648432946
1103	49	-0.44495528314725163
1103	50	-0.5122038939406721
1103	51	-0.41246965852317174
2204	1	-0.42914082941131326
2204	2	-0.4786066334800019
2204	3	-0.45580240970366503
2204	4	-0.5612749079638988
2204	5	-0.5652239140249478
2204	6	-0.6832084658996784
2204	7	-0.7608356319705791
2204	8	-0.7015774245625614
2204	9	-0.7521584459461188
2204	10	-0.675568095330938
2204	11	-0.6904060822018323
2204	12	-0.6682533951142702
2204	13	-0.6692316786331638
2204	14	-0.829990099911516
2204	15	-0.8000007779243141
2204	16	-0.8543901845703346
2204	17	-0.8025073594875707
2204	18	-0.6276694122791753
2204	19	-0.7267918432220313
2204	20	-0.772231778388889
2204	21	-0.7510497230236872
2204	22	-0.7699109967185116
2204	23	-0.8682973791891762
2204	24	-0.7620971842266222
2204	25	-0.7948753637975265
2204	26	-0.7274590386615678
2204	27	-0.813031710637695
2204	28	-0.7296054210035834
2204	29	-0.6846542054919134
2204	30	-0.8438747849891861
2204	31	-0.7386190347487904
2204	32	-0.7499113282218595
2204	33	-0.6346346059426615
2204	34	-0.6355295663148866
2204	35	-0.6974552164757455
2204	36	-0.6213356563696912
2204	37	-0.6694772419902577
2204	38	-0.6507595364327159
2204	39	-0.6507488326832025
2204	40	-0.6359108738744581
2204	41	-0.6025640735299156
2204	42	-0.5484273171757224
2204	43	-0.5363212415324838
2204	44	-0.6036647880384542
2204	45	-0.516230842351387
2204	46	-0.5427688003670781
2204	47	-0.5299502011952164
2204	48	-0.6303488186991331
2204	49	-0.6228601553410087
2204	50	-0.7124168295701949
2204	51	-0.41480051510901816
2205	1	-0.22351581500610962
2205	2	-0.24968074453133582
2205	3	-0.21943809492198696
2205	4	-0.42634185210993536
2205	5	-0.35129342103248007
2205	6	-0.3683028319329651
2205	7	-0.37083897116718134
2205	8	-0.40696296123160636
2205	9	-0.39321687570297215
2205	10	-0.31744270287695997
2205	11	-0.39509918529924803
2205	12	-0.37785515937935443
2205	13	-0.35769334898475336
2205	14	-0.39673123797043175
2205	15	-0.39659729897615037
2205	16	-0.3609867140544871
2205	17	-0.3091163266358343
2205	18	-0.3511240641342006
2205	19	-0.4356785472045033
2205	20	-0.3628755771803682
2205	21	-0.407650314072729
2205	22	-0.40306771522142526
2205	23	-0.4351510183794254
2205	24	-0.3881801042359996
2205	25	-0.3591628388308514
2205	26	-0.3691479947466334
2205	27	-0.43193388229601126
2205	28	-0.31420170426414273
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1205	42	-0.17214669422876128
1205	43	-0.192845901741169
1205	44	-0.30961982660162235
1205	45	-0.2599899017362546
1205	46	-0.2341238556216858
1205	47	-0.18668947563429938
1205	48	-0.25186331912741977
1205	49	-0.25621328793705445
1205	50	-0.14474371784194062
1205	51	-0.12327379640555947
1210	1	-0.3308128562318693
1210	2	-0.17745240179004895
1210	3	-0.5475025366931341
1210	4	-0.4162782181639386
1210	5	-0.2802304431838962
1210	6	-0.21853171572535857
1210	7	-0.2363942321597321
1210	8	-0.2794490327087118
1210	9	-0.1839445185792279
1210	10	-0.2073643338334179
1210	11	-0.22152692663179963
1210	12	-0.2162346904739536
1210	13	-0.13971853078709628
1210	14	-0.22214211168043363
1210	15	-0.15652921323830782
1210	16	-0.09530300552847137
1210	17	-0.1846779068385338
1210	18	-0.08748419908851801
1210	19	-0.004618795654504737
1210	20	0.012105665269718457
1210	21	-0.1280917500840566
1210	22	-0.10703324884871179
1210	23	-0.15674395953824316
1210	24	-0.15625279516076063
1210	25	-0.10781927721396656
1210	26	-0.17504101251962112
1210	27	-0.1396079919584814
1210	28	-0.08743115828605497
1210	29	-0.12457755315884356
1210	30	-0.07223558889271331
1210	31	-0.0947807226914179
1210	32	-0.14486905701282282
1210	33	-0.07814792480466437
1210	34	-0.06951018026767386
1210	35	-0.010084120783105277
1210	36	-0.22141056498348988
1210	37	-0.09573518617320076
1210	38	-0.05492426378850368
1210	39	-0.14121759602197415
1210	40	-0.07472195377714286
1210	41	-0.09156280601240496
1210	42	0.00430966836154667
1210	43	0.019281938860448378
1210	44	-0.12252237085061068
1210	45	-0.1146850149408607
1210	46	-0.08152722610040186
1210	47	-0.10001121948392466
1210	48	-0.03991890100701667
1210	49	-0.12544206942039438
1210	50	-0.28504420550131826
1210	51	-0.18280335036579132
1211	1	-0.28022567518214103
1211	2	-0.25805032701444475
1211	3	-0.30773338701274966
1211	4	-0.4192423135738288
1211	5	-0.4397456230665071
1211	6	-0.4069028816228091
1211	7	-0.4173403172732506
1211	8	-0.3996207774036158
1211	9	-0.33493699968321167
1211	10	-0.3480877951901036
1211	11	-0.46976052048983874
1211	12	-0.38257447284489743
1211	13	-0.35289339134331144
1211	14	-0.2836853115195481
1211	15	-0.30162024998277914
1211	16	-0.28657464268031774
1211	17	-0.2387546904551101
1211	18	-0.4403144862127846
1211	19	-0.4104047439355618
1211	20	-0.3828931519086005
1211	21	-0.35789713412624735
1211	22	-0.32506107225925973
1211	23	-0.4438475559746813
1211	24	-0.4276515590487868
1211	25	-0.3901280640846956
1211	26	-0.4145173695098947
1211	27	-0.3202077914613426
1211	28	-0.2785020956735025
1211	29	-0.3396534386414416
1211	30	-0.49932651115423
1211	31	-0.36715471925591814
1211	32	-0.28044635757566283
1211	33	-0.31739713950731385
1211	34	-0.31135744886140415
1211	35	-0.3345004476734772
1211	36	-0.38181911716416295
1211	37	-0.3574787818396672
1211	38	-0.2830830182139543
1211	39	-0.29119279982442947
1211	40	-0.43163522989939196
1211	41	-0.3730517733508236
1211	42	-0.3658207155666666
1211	43	-0.3535567267951194
1211	44	-0.32995484743605985
1211	45	-0.18876369309837315
1211	46	-0.33009940583053776
1211	47	-0.3278060599499
1211	48	-0.38591972265017205
1211	49	-0.3767160468875126
1211	50	-0.37661901985909907
1211	51	-0.33286699984937584
1208	1	-0.18602475112101047
1208	2	-0.13138775822290327
1208	3	-0.12854981580197133
1208	4	-0.19844135701454135
1208	5	-0.2168367208374029
1208	6	-0.16848038228151196
1208	7	-0.018628461337190316
1208	8	-0.15313960917904623
1208	9	-0.17395284933197885
1208	10	-0.21208644088445183
1208	11	-0.1677243361877423
1208	12	-0.17349353625061426
1208	13	-0.17544827729873447
1208	14	-0.19055349265210353
1208	15	-0.2149176929216452
1208	16	-0.22895288626500587
1208	17	-0.21002240340704859
1208	18	-0.22222107278638248
1208	19	-0.2497191946746362
1208	20	-0.2286640541467655
1208	21	-0.2523030814183791
1208	22	-0.18099700298510157
1208	23	-0.22128781471697678
1208	24	-0.301476883399161
1208	25	-0.3657820894863413
1208	26	-0.25507775555174206
1208	27	-0.22574574924351865
1208	28	-0.3840079437232582
1208	29	-0.3783440154060358
1208	30	-0.29887377040082796
1208	31	-0.30900787808321084
1208	32	-0.2987248227563484
1208	33	-0.35889495494497226
1208	34	-0.30696906481605524
1208	35	-0.24806829858074053
1208	36	-0.27847569127581906
1208	37	-0.3399006778287337
1208	38	-0.2334734168860635
1208	39	-0.31990349696167897
1208	40	-0.26573294965523075
1208	41	-0.4136518757653356
1208	42	-0.2571146730458729
1208	43	-0.3588025895920488
1208	44	-0.44899876720575094
1208	45	-0.274395397056999
1208	46	-0.269658434329866
1208	47	-0.2603415052749867
1208	48	-0.38126248433316334
1208	49	-0.43374872566136347
1208	50	-0.47595287238609174
1208	51	-0.29933848336433044
1209	1	0.035529161462296344
1209	2	0.08781854025274427
1209	3	0.03130319177056862
1209	4	0.11998831898026197
1209	5	0.16670595986079917
1209	6	0.17524570954072516
1209	7	0.14976695975917664
1209	8	0.09046282176832321
1209	9	0.10985894825872577
1209	10	0.05174750263324768
1209	11	0.13620179443450198
1209	12	0.12228537890071323
1209	13	0.0682308484111509
1209	14	0.054745778483621166
1209	15	0.1676894059707159
1209	16	0.059112956062882915
1209	17	0.12460247182251649
1209	18	0.16499459552612095
1209	19	0.06980297306636629
1209	20	0.04184431394333288
1209	21	0.19995131712054715
1209	22	0.1121782821402526
1209	23	0.1081014171120529
1209	24	0.12416597272888197
1209	25	-0.007675446802085872
1209	26	0.0629953840942079
1209	27	0.18842143301731085
1209	28	0.10867128973647766
1209	29	0.1688283870020797
1209	30	0.03483334512549874
1209	31	0.1403528962170526
1209	32	0.28020747802240464
1209	33	0.1114275037834993
1209	34	0.12735403538238188
1209	35	0.24580988775660373
1209	36	0.024524255792222505
1209	37	0.077509215569318
1209	38	0.03414824206691236
1209	39	0.1242418448939091
1209	40	0.13096898067650642
1209	41	0.059001160775192574
1209	42	0.10006204564289334
1209	43	0.014653189653770937
1209	44	0.019279776101704726
1209	45	0.13523041799772884
1209	46	0.17147076026071417
1209	47	0.2719209638160791
1209	48	0.09658369916571985
1209	49	0.1433627345855406
1209	50	0.16583650694834162
1209	51	0.10126579592681395
1214	1	0.18352725902215639
1214	2	0.21469432244263942
1214	3	0.13901703050389003
1214	4	0.2140890490399805
1214	5	0.18432174968531712
1214	6	0.24176424193547064
1214	7	0.26674905501474555
1214	8	0.24113083295013382
1214	9	0.23102202101738456
1214	10	0.16469853262001166
1214	11	0.222301846949712
1214	12	0.28567939555928135
1214	13	0.20396967092464635
1214	14	0.29577969646344116
1214	15	0.3536481533449951
1214	16	0.31739397652795276
1214	17	0.2520509885594535
1214	18	0.2626750007080254
1214	19	0.16558791931214145
1214	20	0.26951114955724975
1214	21	0.21263111802999646
1214	22	0.16265250052753188
1214	23	0.17877012396501613
1214	24	0.11610240308281305
1214	25	0.22146938381775882
1214	26	0.09942739453270377
1214	27	0.23006007661900796
1214	28	0.22353091435146766
1214	29	0.2845621322184577
1214	30	0.27150740748280455
1214	31	0.14345227654613524
1214	32	0.1853441429571383
1214	33	0.19054500205940172
1214	34	0.1249596957205128
1214	35	0.2944591022835503
1214	36	0.15772507275265824
1214	37	0.19034110574733631
1214	38	0.25711188752337577
1214	39	0.3191489686614304
1214	40	0.27781481165125754
1214	41	0.20692763972475703
1214	42	0.18879471743746024
1214	43	0.16312799954524948
1214	44	0.13505550041800518
1214	45	0.24913135142490717
1214	46	0.1487583525217282
1214	47	0.1034476597831997
1214	48	0.07451423609494157
1214	49	0.1448943746556992
1214	50	0.06333061800936335
1214	51	0.09910544917638475
1215	1	0.6097561911604608
1215	2	0.6887390978669607
1215	3	0.6838942748847359
1215	4	0.8921594213076709
1215	5	0.8308276916584667
1215	6	0.7901376611843318
1215	7	0.8480935455122456
1215	8	0.7778944368298824
1215	9	0.9128309464664746
1215	10	0.8645116155451475
1215	11	0.8823322871256636
1215	12	0.7093710818772898
1215	13	0.8386337917370064
1215	14	0.9030402499750707
1215	15	0.8084974130856395
1215	16	0.8933216821684908
1215	17	0.7946096568123409
1215	18	0.7710794951851874
1215	19	0.8058695015239046
1215	20	0.8042643893826558
1215	21	0.8463712376738606
1215	22	0.9452954613956059
1215	23	0.9108485232944012
1215	24	0.7305149161543092
1215	25	0.8449479042304553
1215	26	0.7810737465803221
1215	27	0.7980269776905544
1215	28	0.7669695534766205
1215	29	0.889514058755605
1215	30	0.9118804372328881
1215	31	0.7891385776202213
1215	32	0.7091234664993777
1215	33	0.787000351528711
1215	34	0.8332312525725172
1215	35	0.8308785077898797
1215	36	0.7553969708394597
1215	37	0.7854033620459973
1215	38	0.9095024362509427
1215	39	0.8308394356789286
1215	40	0.7723916762707965
1215	41	0.7331821856411587
1215	42	0.8495326059307367
1215	43	0.7384938955406071
1215	44	0.8399733458123961
1215	45	0.7720884938586821
1215	46	0.7408142709979408
1215	47	0.8451621317795066
1215	48	0.8628462075937939
1215	49	0.8369479646066296
1215	50	0.8729129229106967
1215	51	0.7652965321946645
1212	1	0.9548881081273315
1212	2	1.0503955773252152
1212	3	1.026197149941332
1212	4	1.1975940755331465
1212	5	1.339576141431099
1212	6	1.22606233489838
1212	7	1.3508641463114728
1212	8	1.2441931090478384
1212	9	1.4474984596064964
1212	10	1.4557760843339054
1212	11	1.2523643005511254
1212	12	1.2828886461994244
1212	13	1.3012952122374095
1212	14	1.4957269908557969
1212	15	1.333470345623681
1212	16	1.2806037890997288
1212	17	1.3968402617455098
1212	18	1.3500560744485561
1212	19	1.3995129541509215
1212	20	1.3625541687090212
1212	21	1.319957454922232
1212	22	1.266726247434704
1212	23	1.3835354856361803
1212	24	1.4517023411036192
1212	25	1.4010107696411929
1212	26	1.3542620721379137
1212	27	1.3324999742062325
1212	28	1.263110594638846
1212	29	1.3368063282884677
1212	30	1.4317079682866485
1212	31	1.3914615810258013
1212	32	1.2806230284986277
1212	33	1.304669375525247
1212	34	1.427172816382825
1212	35	1.40961074169212
1212	36	1.3303673196652213
1212	37	1.2476433913772844
1212	38	1.4330435000514115
1212	39	1.3193004536123532
1212	40	1.4583073685088337
1212	41	1.447906638878429
1212	42	1.4089120966687432
1212	43	1.3308726825246033
1212	44	1.4161975332964012
1212	45	0.6565590296960018
1212	46	0.9398692474738546
1212	47	1.0096790267971265
1212	48	1.3132356099442326
1212	49	1.2446774474704654
1212	50	1.3046646552282617
1212	51	1.2391450577561898
1213	1	1.9494652792417781
1213	2	2.1294710309499436
1213	3	2.089221472402315
1213	4	2.491304646484082
1213	5	2.6154261920711157
1213	6	2.472951482071693
1213	7	2.5672484033675183
1213	8	2.513043931490518
1213	9	2.5735051587315816
1213	10	2.5223818151974697
1213	11	2.4685891635790327
1213	12	2.5893923038729127
1213	13	2.5461160502991795
1213	14	2.6954976617445006
1213	15	2.6834599735720914
1213	16	2.6859027634868653
1213	17	2.6126117288416992
1213	18	2.698913547206672
1213	19	2.668144895798978
1213	20	2.6224983731726645
1213	21	2.6292806327968172
1213	22	2.6860675306405213
1213	23	2.6513921926928354
1213	24	2.706302651044922
1213	25	2.8200515684111878
1213	26	2.525558410803704
1213	27	2.57815782157844
1213	28	2.6411081938139667
1213	29	2.66887366120692
1213	30	2.752668435517066
1213	31	2.5792151624342026
1213	32	2.6614157430475665
1213	33	2.6712180601050584
1213	34	2.631371819445633
1213	35	2.6910506417543516
1213	36	2.544947144804638
1213	37	2.6283125564881438
1213	38	2.598501365117123
1213	39	2.6500068360848097
1213	40	2.5512009116016543
1213	41	2.735637264496173
1213	42	2.6437235236189913
1213	43	2.6658624935092163
1213	44	2.616078407233264
1213	45	2.656684485761211
1213	46	2.6487429189360725
1213	47	2.542898953948715
1213	48	2.594177498999887
1213	49	2.6706362521220264
1213	50	2.7073576516529556
1213	51	2.3178654974091515
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	614.0
3	136.0
4	99.0
5	22.0
6	5841.0
7	37638.0
8	127161.0
9	190439.0
10	170595.0
11	139686.0
12	113470.0
13	80665.0
14	54994.0
15	37850.0
16	27376.0
17	20902.0
18	17447.0
19	15070.0
20	13633.0
21	12864.0
22	12480.0
23	12304.0
24	12384.0
25	12878.0
26	13658.0
27	14671.0
28	16474.0
29	20618.0
30	19996.0
31	23546.0
32	30410.0
33	34966.0
34	46819.0
35	69736.0
36	116544.0
37	296808.0
38	1513992.0
39	98343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.729326894639474	31.988202656267163	20.6246345017525	26.657835947340864
2	22.24694175859042	36.85852827403801	22.40377614549849	18.490753821873078
3	21.652432442668584	35.102426688769405	23.069144387908946	20.175996480653062
4	22.612505054672035	33.39410593194168	22.81128330985169	21.1821057035346
5	24.106575784025928	32.04554115298513	21.286284979775154	22.561598083213788
6	20.929362647261296	32.77823240708398	22.352486709011767	23.939918236642953
7	19.950750971928287	42.01157107429541	20.93951902368988	17.098158930086424
8	31.539993817544133	30.84407420928225	21.202327423678575	16.413604549495044
9	18.083691789897642	43.69572893807898	20.982557509407666	17.23802176261571
10	20.52420500284799	31.062205640914332	30.829355130534097	17.584234225703586
11	18.600379967037668	32.90257583389829	20.498088365497875	27.998955833566164
12	32.406989533181346	29.47986393864128	21.00260031611686	17.110546212060516
13	30.321909969629374	30.571453711019895	20.323290982278753	18.783345337071975
14	19.490904196149337	43.38251789937889	20.148724944220135	16.97785296025164
15	18.33244858608455	42.95399333729964	21.47272832160436	17.240829755011447
16	29.98282162400917	30.748156733451392	20.95709426721232	18.31192737532712
17	19.445124282091445	41.90327930372194	20.135878179309316	18.515718234877305
18	31.293897863708924	29.718258277279354	21.581367595724913	17.40647626328681
19	20.18455784812262	31.762456940921034	20.491853191667275	27.56113201928907
20	19.25543293468358	42.32172133714861	20.978862054869676	17.44398367329814
21	18.507843975912742	33.19465600369211	21.267628696330153	27.029871324064995
22	20.430987237680178	41.43705533426976	19.947858713896835	18.18409871415323
23	20.378207940944925	31.287406917923445	20.139467965896955	28.194917175234675
24	31.54222408172922	29.174013726424825	21.696299645826073	17.587462546019886
25	19.585599534284594	31.912576967308144	28.88869011424071	19.613133384166552
26	19.230420714802886	32.937020251243034	22.002543606239264	25.83001542771482
27	20.138489683928736	33.39885069762008	28.678104137645903	17.784555480805288
28	29.39813587322306	30.69836284333235	20.778253990915367	19.125247292529217
29	19.701275627193567	39.84576629409643	21.254392962635563	19.19856511607444
30	21.0575509970992	37.005136130175906	22.781307143381593	19.156005729343306
31	20.41868326505234	34.70982107087092	22.15031375459225	22.72118190948449
32	26.537597201992803	32.18733874936374	23.022897845290878	18.252166203352584
33	23.04306655050945	35.907934722752344	21.641895848933462	19.407102877804743
34	22.13947213922448	35.463200234255154	23.631654164803162	18.7656734617172
35	22.42255658332049	34.92273504283465	21.730425030833434	20.924283343011425
36	25.575850565995072	33.081699031233676	21.77085904040591	19.57159136236534
37	25.356160136869914	32.834648927032624	22.360703043327767	19.44848789276969
38	24.903005913463446	34.082043769419215	20.707727593754154	20.307222723363193
39	22.136435523327027	35.083370336313386	22.47665898636309	20.303535153996496
40	25.57688133430258	33.28510389093891	21.185360431559303	19.952654343199196
41	24.008381874080754	35.01364152337531	22.175429640973285	18.802546961570645
42	27.282249436510398	31.38316593749053	21.222347053654467	20.112237572344608
43	24.021687635809947	31.665416734407753	23.248419520387298	21.064476109395002
44	27.58176215065581	30.02268819738819	22.516120216270647	19.879429435685356
45	25.397293433928752	30.178712845612214	24.987806292244567	19.436187428214463
46	22.821980319584586	31.713713560303287	24.058612942409454	21.405693177702677
47	24.99793860570495	32.90145544634941	21.45003864932201	20.650567298623628
48	27.62831070956253	30.46256333844868	22.841816385439245	19.067309566549547
49	27.424277034925392	30.743133390596356	22.398893277499944	19.433696296978304
50	24.361774813636412	31.26421309185099	24.310203415658183	20.063808678854418
51	24.015943498479075	35.49776814330502	21.7541344708227	18.732153887393217
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5344.0
1	5547.5
2	5751.0
3	5558.0
4	5365.0
5	5022.0
6	4679.0
7	4528.5
8	4378.0
9	4302.0
10	4226.0
11	4561.0
12	4896.0
13	5246.5
14	5597.0
15	6430.5
16	7264.0
17	8307.5
18	9351.0
19	11027.5
20	12704.0
21	15262.0
22	17820.0
23	21333.0
24	24846.0
25	30941.5
26	45477.5
27	53918.0
28	60807.5
29	67697.0
30	81498.5
31	95300.0
32	107527.5
33	119755.0
34	141911.0
35	164067.0
36	186230.5
37	208394.0
38	232846.0
39	257298.0
40	270565.5
41	283833.0
42	305641.5
43	327450.0
44	332740.0
45	338030.0
46	322709.0
47	307388.0
48	277754.5
49	248121.0
50	228195.5
51	208270.0
52	187856.0
53	167442.0
54	149609.0
55	131776.0
56	123597.5
57	115419.0
58	87858.0
59	60297.0
60	52341.5
61	44386.0
62	34140.5
63	23895.0
64	19977.0
65	16059.0
66	13568.5
67	11078.0
68	9237.5
69	7397.0
70	6655.5
71	5914.0
72	5142.0
73	4370.0
74	3687.0
75	2519.0
76	2034.0
77	1731.5
78	1429.0
79	1312.0
80	1195.0
81	1070.0
82	945.0
83	845.5
84	746.0
85	689.0
86	632.0
87	535.0
88	438.0
89	373.5
90	309.0
91	281.0
92	253.0
93	210.5
94	168.0
95	164.0
96	160.0
97	197.5
98	235.0
99	2502.0
100	4769.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03407969814125831
2	0.024642243271371392
3	0.019632236365135128
4	0.01925357305245448
5	0.020884738091694194
6	0.01572909144981153
7	0.022632414919451033
8	0.024350963800078586
9	0.023914044593139377
10	0.025749105262284053
11	0.02114688961585772
12	0.024875266848405637
13	0.025195674266827724
14	0.025836489103671898
15	0.025836489103671898
16	0.02641904804625751
17	0.027292886460135928
18	0.025399569896732692
19	0.02697247904171384
20	0.027962829244109384
21	0.02740939824865305
22	0.026681199570421036
23	0.027467654142911614
24	0.026710327517550318
25	0.028836667657987803
26	0.028953179446504922
27	0.027962829244109384
28	0.027933701296980102
29	0.027234630565877365
30	0.028341492556790034
31	0.02877841176372924
32	0.028312364609660752
33	0.029098819182151327
34	0.028370620503919308
35	0.028487132292436435
36	0.030060041437417584
37	0.028108468979755784
38	0.027991957191238665
39	0.028341492556790034
40	0.026914223147455278
41	0.027991957191238665
42	0.027525910037170177
43	0.027467654142911614
44	0.027613293878558015
45	0.030030913490288302
46	0.028720155869470677
47	0.028720155869470677
48	0.028312364609660752
49	0.02950661044196125
50	0.028953179446504922
51	0.029390098653444132
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3433129.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.90195107449158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.55913588204645	46.25371002816973
2	1.737048942850825	1.6641606694887507
3	0.489052782750346	0.7027974741645311
4	0.1540135736647645	0.2951020268198861
5	0.0806649109730768	0.1932003309430272
6	0.07918525976007301	0.2275877063308755
7	0.030582267795646562	0.10254652069858426
8	0.020839756776847792	0.07986120076230936
9	0.010598910104331893	0.045693762593457664
>10	0.11220509269194279	1.2173322847805874
>50	0.19876619628648534	7.660893728737039
>100	0.5270826255493131	40.52094056957509
>500	6.410187962864651E-4	0.17150998151599622
>1k	1.2185355389214927E-4	0.2712756349897769
>5k	0.0	0.0
>10k+	6.0926399573620636E-5	0.5933880804303482
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	20332	0.5922294210325333	Illumina Single End PCR Primer 1 (100% over 51bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC	4740	0.13806646939279008	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4555	0.13267779917387315	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.8710421309540073	0.0	0.0
2	0.8745083566623917	0.0	0.0
3	0.8750326597107188	0.0	0.0
4	0.8769259762741219	0.0	0.0
5	0.8833049966954344	0.0	0.0
6	0.9651836560758422	0.0	0.0
7	7.064867064418494	0.0	0.0
8	7.072585970407753	0.0	0.0
9	7.077333825789826	0.0	0.0
10	7.106986075967434	0.0	0.0
11	7.212574884311076	0.0	0.0
12	7.331183885021507	0.0	0.0
13	7.794085220800034	0.0	0.0
14	7.823300551770703	0.0	0.0
15	7.8539431521507055	0.0	0.0
16	7.874507482823978	0.0	0.0
17	7.897372921320463	0.0	0.0
18	7.9107426490528026	0.0	0.0
19	7.924520168044952	0.0	0.0
20	7.937103441204802	0.0	0.0
21	7.947968165484023	0.0	0.0
22	7.959881495859899	0.0	0.0
23	7.970513196562087	0.0	0.0
24	7.9831838535633235	0.0	0.0
25	8.006660978949524	0.0	0.0
26	8.074383456025101	0.0	0.0
27	8.226926515141145	0.0	0.0
28	8.263365576999874	0.0	0.0
29	8.28899817047364	0.0	0.0
30	8.340117717685528	0.0	0.0
31	8.557790866582643	0.0	0.0
32	8.688080174091914	0.0	0.0
33	8.78944543010181	0.0	0.0
34	8.834185956892386	0.0	0.0
35	8.867857863773834	0.0	0.0
36	8.894422551555738	0.0	0.0
37	8.913646996661063	0.0	0.0
38	8.932958825607777	0.0	0.0
39	8.953406644492532	2.9127947129280607E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGTCT	11855	0.0	43.747505	21
ACGTCTG	11450	0.0	43.703197	22
CACACGT	18395	0.0	43.65467	19
GCACACG	20495	0.0	43.583942	18
CGTCTGA	11190	0.0	43.57253	23
ACACGTC	16080	0.0	43.530926	20
AGCACAC	21305	0.0	42.750668	17
AGAGCAC	22320	0.0	42.68159	15
GAGCACA	22775	0.0	41.937565	16
CACGTCA	4200	0.0	41.57156	21
CTGAAAG	7330	0.0	41.163162	26
GTCTGAA	11845	0.0	41.12508	24
ACACGTA	3695	0.0	40.676723	20
GGGCGCC	1295	0.0	40.135265	44
TCTGAAA	7940	0.0	39.36096	25
CACGTAG	2325	0.0	39.193676	21
CACGGCT	335	0.0	38.95535	21
AAGAGCA	26765	0.0	38.636406	14
ACGTATG	1075	0.0	38.30245	22
ACGACTG	925	0.0	37.946068	22
>>END_MODULE