FastQCFastQC Report
Fri 19 Dec 2014
lane2_Undetermined_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433129
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG203320.5922294210325333Illumina Single End PCR Primer 1 (100% over 51bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC47400.13806646939279008No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45550.13267779917387315No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT118550.043.74750521
ACGTCTG114500.043.70319722
CACACGT183950.043.6546719
GCACACG204950.043.58394218
CGTCTGA111900.043.5725323
ACACGTC160800.043.53092620
AGCACAC213050.042.75066817
AGAGCAC223200.042.6815915
GAGCACA227750.041.93756516
CACGTCA42000.041.5715621
CTGAAAG73300.041.16316226
GTCTGAA118450.041.1250824
ACACGTA36950.040.67672320
GGGCGCC12950.040.13526544
TCTGAAA79400.039.3609625
CACGTAG23250.039.19367621
CACGGCT3350.038.9553521
AAGAGCA267650.038.63640614
ACGTATG10750.038.3024522
ACGACTG9250.037.94606822
Produced by FastQC (version 0.11.2)