FastQCFastQC Report
Fri 19 Dec 2014
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433129
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC138970.4047910812556126TruSeq Adapter, Index 14 (97% over 38bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC39180.11412329685252141No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4350.032.5585217
CCTACGC1000.031.49664145
GGGTGAC3500.030.1828985
TCGTGAC2850.029.21167421
GGTGACT6800.028.4184556
TACACGA5300.027.9828313
GTCGTGA3500.027.0053920
AGTTCAG290150.026.5807421
GTGACTG40250.026.383051
AAGGGTG2050.026.3140983
CGACGCT5800.025.96929617
TGAAGTT13000.025.0942121
GAAGTTC14250.024.9214522
TGTAGTT6450.024.4167141
GAGTGAC2500.024.2747545
TGACTGG42550.023.5595192
AGGGTGA3750.023.375694
GCTTGAA7100.022.79751616
ACTGGAG42900.022.7909854
CACGCAC2450.022.11068532
Produced by FastQC (version 0.11.2)