##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	lane1_Undetermined_L001_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3240789
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.335127649470547	33.0	27.0	33.0	15.0	33.0
2	28.21265346185759	33.0	27.0	33.0	15.0	33.0
3	28.15024119126546	33.0	27.0	33.0	15.0	33.0
4	31.261961824728484	37.0	33.0	37.0	15.0	37.0
5	31.341664329272902	37.0	33.0	37.0	15.0	37.0
6	30.982317577602245	37.0	33.0	37.0	6.0	37.0
7	31.342648657471994	37.0	33.0	37.0	15.0	37.0
8	31.10346523639768	37.0	33.0	37.0	6.0	37.0
9	31.441847648828727	37.0	33.0	37.0	15.0	37.0
10	31.27891911506735	37.0	33.0	37.0	6.0	37.0
11	31.210941224498107	37.0	33.0	37.0	6.0	37.0
12	31.129927002344182	37.0	33.0	37.0	6.0	37.0
13	31.184450761836082	37.0	33.0	37.0	6.0	37.0
14	32.96780290231792	40.0	33.0	40.0	15.0	40.0
15	33.022771615183835	40.0	33.0	40.0	15.0	40.0
16	32.71523076633499	40.0	33.0	40.0	6.0	40.0
17	32.94039105909086	40.0	33.0	40.0	6.0	40.0
18	32.68277385537905	40.0	33.0	40.0	6.0	40.0
19	32.71152333582964	40.0	33.0	40.0	6.0	40.0
20	32.860289886197464	40.0	33.0	40.0	6.0	40.0
21	32.74876796977526	40.0	33.0	40.0	6.0	40.0
22	32.946427552055994	40.0	33.0	40.0	6.0	40.0
23	32.674596834289424	40.0	33.0	40.0	6.0	40.0
24	32.52423005632271	40.0	33.0	40.0	6.0	40.0
25	32.390789094877825	40.0	33.0	40.0	6.0	40.0
26	32.405605857092205	40.0	33.0	40.0	6.0	40.0
27	32.32519735163258	40.0	33.0	40.0	6.0	40.0
28	32.27235281284897	37.0	33.0	40.0	6.0	40.0
29	32.415116195469686	40.0	33.0	40.0	6.0	40.0
30	32.45596890140025	40.0	33.0	40.0	6.0	40.0
31	32.31856964461432	40.0	33.0	40.0	6.0	40.0
32	32.24134616601081	40.0	33.0	40.0	6.0	40.0
33	32.154198560906	37.0	33.0	40.0	6.0	40.0
34	32.12296604314567	40.0	33.0	40.0	6.0	40.0
35	32.11350507546156	40.0	27.0	40.0	6.0	40.0
36	32.10054588558527	37.0	33.0	40.0	6.0	40.0
37	32.112336841429666	37.0	33.0	40.0	6.0	40.0
38	32.03228812489798	37.0	27.0	40.0	6.0	40.0
39	32.002421323943025	37.0	27.0	40.0	6.0	40.0
40	31.936988184050243	37.0	27.0	40.0	6.0	40.0
41	31.932836108737717	37.0	27.0	40.0	6.0	40.0
42	31.811237633798434	37.0	27.0	40.0	6.0	40.0
43	31.753317787736258	37.0	27.0	40.0	6.0	40.0
44	31.777915192874328	37.0	27.0	40.0	6.0	40.0
45	31.62174303850081	37.0	27.0	40.0	6.0	40.0
46	31.562177914082035	37.0	27.0	40.0	6.0	40.0
47	31.640806606045626	37.0	27.0	40.0	6.0	40.0
48	31.55138177770907	37.0	27.0	40.0	6.0	40.0
49	31.466750843698865	37.0	27.0	40.0	6.0	40.0
50	31.398226172700536	37.0	27.0	40.0	6.0	40.0
51	30.61630393092546	37.0	27.0	40.0	6.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	-0.22724808569808985
2201	2	-0.26821636438447527
2201	3	-0.498652531053402
2201	4	-0.5147775608613472
2201	5	-0.6210416178009375
2201	6	-0.4851856000708814
2201	7	-0.5756523058298804
2201	8	-0.40043941127122906
2201	9	-0.3206440559489643
2201	10	-0.25282238948782876
2201	11	-0.14782077326198362
2201	12	-0.20773075212959213
2201	13	-0.2244498305501672
2201	14	-0.18854797537874646
2201	15	-0.20206719090645464
2201	16	-0.2052316943771615
2201	17	-0.30826110454879796
2201	18	-0.13599061844265492
2201	19	-0.14457094798932246
2201	20	-0.17054539005219027
2201	21	-0.22414184328678033
2201	22	-0.28516843040852535
2201	23	-0.13366759230934377
2201	24	-0.11104417230957608
2201	25	-0.052709447510295604
2201	26	-0.09404799716345025
2201	27	-0.18687201536370424
2201	28	-0.1571776409289356
2201	29	-0.14660877446693377
2201	30	-0.21764645555113304
2201	31	-0.1227209064349779
2201	32	-0.05209573570067505
2201	33	-0.16024133064725987
2201	34	-0.07359252992736742
2201	35	-0.12184709014891837
2201	36	-0.09043470342511739
2201	37	-0.13596977791755194
2201	38	0.009069504913149729
2201	39	-0.03078069174352649
2201	40	-0.06586306683762544
2201	41	-0.11032871617659623
2201	42	-0.2838744302416103
2201	43	-0.25945076315163007
2201	44	-0.09849035751287616
2201	45	-0.13945490070718236
2201	46	-0.06651682412599413
2201	47	-0.04823598319950051
2201	48	-0.006943036034147809
2201	49	-0.24043303006590122
2201	50	-0.1878408154679434
2201	51	-0.3194329904103448
1101	1	-0.27632758683632375
1101	2	-0.2341715736589478
1101	3	-0.1717694016146183
1101	4	-0.3304586966336238
1101	5	-0.3504442342447902
1101	6	-0.592140967230609
1101	7	-0.5208602796612674
1101	8	-0.45578368806981473
1101	9	-0.3963161637449488
1101	10	-0.29899193321335105
1101	11	-0.34744393523764927
1101	12	-0.4017249917521113
1101	13	-0.41830218210151315
1101	14	-0.4025452336004278
1101	15	-0.32673853570629063
1101	16	-0.3591653672681261
1101	17	-0.48644553539331525
1101	18	-0.3369592131873702
1101	19	-0.3209232143382721
1101	20	-0.3381291127083088
1101	21	-0.3676499423830748
1101	22	-0.430795530473695
1101	23	-0.2284300445525318
1101	24	-0.34504695303755994
1101	25	-0.34103087685177513
1101	26	-0.41783824486246246
1101	27	-0.39752544470796636
1101	28	-0.3818852228293039
1101	29	-0.4754947514045611
1101	30	-0.4324609612416097
1101	31	-0.37800272466105866
1101	32	-0.316606677427103
1101	33	-0.2761638847994661
1101	34	-0.2376823283837055
1101	35	-0.34603747611924973
1101	36	-0.4037526377295464
1101	37	-0.3717531148328632
1101	38	-0.40903221353223174
1101	39	-0.43568709778128323
1101	40	-0.37154895307816815
1101	41	-0.3456239303058659
1101	42	-0.5646124986024503
1101	43	-0.3895962053798847
1101	44	-0.4439644410307011
1101	45	-0.4528632538805013
1101	46	-0.38107307961633197
1101	47	-0.26452389913499275
1101	48	-0.2858298823839718
1101	49	-0.43906714872188957
1101	50	-0.3794204539733066
1101	51	-0.5601875567371053
2202	1	-0.3256320681281828
2202	2	-0.24646603247674648
2202	3	-0.2852536190122379
2202	4	-0.32797644378262447
2202	5	-0.3244970414703232
2202	6	-0.2790577238585179
2202	7	-0.6090668301211721
2202	8	-0.4201870480020169
2202	9	-0.43056908358337154
2202	10	-0.46522414956265123
2202	11	-0.4079908025496941
2202	12	-0.4490404393901848
2202	13	-0.4509176746217989
2202	14	-0.46393504986895806
2202	15	-0.4888460371693384
2202	16	-0.42614358223022464
2202	17	-0.5903452991186171
2202	18	-0.4807413055041252
2202	19	-0.5866901862094878
2202	20	-0.6422968332328054
2202	21	-0.6157560419977912
2202	22	-0.6614542298378296
2202	23	-0.5429521185374213
2202	24	-0.48810477841141875
2202	25	-0.37020079205524326
2202	26	-0.6073894858790396
2202	27	-0.4251138637234888
2202	28	-0.4607152534339747
2202	29	-0.45316605559493794
2202	30	-0.5662280858020665
2202	31	-0.5431245570344565
2202	32	-0.46890911306762817
2202	33	-0.5165957024174794
2202	34	-0.546615042501756
2202	35	-0.29479934306192845
2202	36	-0.4530609179443239
2202	37	-0.516949321878375
2202	38	-0.40651934044710814
2202	39	-0.5958925288862602
2202	40	-0.47878481190799604
2202	41	-0.4708270925842264
2202	42	-0.5088357511413868
2202	43	-0.4909341219535257
2202	44	-0.5720271853231864
2202	45	-0.6244764843481541
2202	46	-0.6006299988784178
2202	47	-0.5639905669733736
2202	48	-0.5566632900938089
2202	49	-0.5732957279915425
2202	50	-0.5785616868510353
2202	51	-0.4505943975811846
1102	1	-0.37116059739147644
1102	2	-0.43071277445413614
1102	3	-0.3998130516273868
1102	4	-0.3922477957758339
1102	5	-0.5106897320594577
1102	6	-0.5010324431971931
1102	7	-0.6933684636170483
1102	8	-0.5518598362046312
1102	9	-0.49313404042814923
1102	10	-0.516215435512386
1102	11	-0.5000234401679045
1102	12	-0.5409097569415273
1102	13	-0.5529543392387879
1102	14	-0.5871271766088029
1102	15	-0.643264769677117
1102	16	-0.5450582166285756
1102	17	-0.526488802670066
1102	18	-0.6306695610217972
1102	19	-0.6716642017350551
1102	20	-0.6296355774586502
1102	21	-0.6833689868105424
1102	22	-0.6561508860396259
1102	23	-0.5948945854188779
1102	24	-0.6341496456724052
1102	25	-0.5560157018836343
1102	26	-0.5153192525197596
1102	27	-0.6026700398354841
1102	28	-0.6296357833307518
1102	29	-0.610401429766334
1102	30	-0.684475895047008
1102	31	-0.5634165326793621
1102	32	-0.7522670847800015
1102	33	-0.7134723559137726
1102	34	-0.6589447785101932
1102	35	-0.7518941803591943
1102	36	-0.8014817492148119
1102	37	-0.7467397008764358
1102	38	-0.725753720979565
1102	39	-0.6618043749482823
1102	40	-0.6116558883843304
1102	41	-0.47293358282470166
1102	42	-0.6308609338627669
1102	43	-0.5852981296491038
1102	44	-0.6622324789077183
1102	45	-0.6472692279707282
1102	46	-0.6915756594008577
1102	47	-0.6477245308269346
1102	48	-0.6468671076330814
1102	49	-0.8738636928429102
1102	50	-0.6444297725123675
1102	51	-0.7379083442466339
2203	1	-0.658931215773503
2203	2	-0.612257511641058
2203	3	-0.6957621808961711
2203	4	-0.7975413535929867
2203	5	-0.847966935401498
2203	6	-0.8432291741916274
2203	7	-0.9075469013900701
2203	8	-0.8472830394653386
2203	9	-0.9075518640786377
2203	10	-0.9005472848011493
2203	11	-0.9021126857532522
2203	12	-0.9680640758069643
2203	13	-0.8388108153291576
2203	14	-0.8255560445411589
2203	15	-0.8927990803521695
2203	16	-1.0290284815180009
2203	17	-0.9461543032136959
2203	18	-1.0403155221244695
2203	19	-0.9222283481547606
2203	20	-0.8584139660236616
2203	21	-0.8752476114343501
2203	22	-0.9152306469659166
2203	23	-0.9416820376228223
2203	24	-0.9629327168026229
2203	25	-0.9077059230855511
2203	26	-0.9098773313207573
2203	27	-0.988336502358738
2203	28	-0.9639194309504688
2203	29	-1.1197478299211063
2203	30	-1.1155211791493294
2203	31	-1.0156929781851858
2203	32	-1.043118913219029
2203	33	-0.9966191876038941
2203	34	-0.9238344116804988
2203	35	-0.9992453887690829
2203	36	-1.031610606015807
2203	37	-0.9279488952985986
2203	38	-1.0118761996338428
2203	39	-0.9617251410159504
2203	40	-1.0378858935228301
2203	41	-0.8913000411993686
2203	42	-1.034228642975247
2203	43	-0.9674893483606333
2203	44	-1.0724366510733176
2203	45	-0.892013890565206
2203	46	-0.9452914186562147
2203	47	-0.9185506708775328
2203	48	-0.9283454674044265
2203	49	-0.9413995914826252
2203	50	-0.9672774868660312
2203	51	-0.831560552055592
1103	1	-0.2998023208317697
1103	2	-0.2526419872984782
1103	3	-0.2573899154093269
1103	4	-0.2392024508029742
1103	5	-0.28286398001659663
1103	6	-0.4150721629512333
1103	7	-0.3223886177314874
1103	8	-0.3780733378621157
1103	9	-0.43608951991083345
1103	10	-0.34409725348609044
1103	11	-0.3630051318886487
1103	12	-0.37955979468121015
1103	13	-0.37313301995730086
1103	14	-0.38661344267114117
1103	15	-0.41025654981923765
1103	16	-0.3121937103340997
1103	17	-0.4454390708847882
1103	18	-0.4354062337118805
1103	19	-0.4248724439602256
1103	20	-0.36092403458052047
1103	21	-0.3706118418915594
1103	22	-0.3635782146896105
1103	23	-0.5076538882977317
1103	24	-0.3683209484656018
1103	25	-0.40398324419790654
1103	26	-0.4757599505926997
1103	27	-0.4980484938943661
1103	28	-0.557380558544768
1103	29	-0.4852372545587009
1103	30	-0.44506103694353527
1103	31	-0.39039881779804375
1103	32	-0.3473021606536264
1103	33	-0.41493444347164044
1103	34	-0.44257931134921336
1103	35	-0.5308738378540419
1103	36	-0.47840701250027706
1103	37	-0.5164514950789432
1103	38	-0.44582472524403727
1103	39	-0.35510412287723625
1103	40	-0.33911105389616125
1103	41	-0.4592272965891304
1103	42	-0.449877885499113
1103	43	-0.4313565749153234
1103	44	-0.39782778906193883
1103	45	-0.3521934462456606
1103	46	-0.4927218725053848
1103	47	-0.5101051527739457
1103	48	-0.3590023990811524
1103	49	-0.5511140449883207
1103	50	-0.47250761201217273
1103	51	-0.5645705866853028
2204	1	-0.04040041060009614
2204	2	-0.11138624326076396
2204	3	-0.18010825982708667
2204	4	-0.21829532055634004
2204	5	-0.24801102281020349
2204	6	-0.24471730492163601
2204	7	-0.15625626056874253
2204	8	-0.30993377684531964
2204	9	-0.33658812055171694
2204	10	-0.27594036741444583
2204	11	-0.29222807189751876
2204	12	-0.28969762271126953
2204	13	-0.22304306201628066
2204	14	-0.23685326233656667
2204	15	-0.3372019669933266
2204	16	-0.41327156937119724
2204	17	-0.38500615145182593
2204	18	-0.29527481354585206
2204	19	-0.35943559149918514
2204	20	-0.24167123050835926
2204	21	-0.2607320128032242
2204	22	-0.44746504743136484
2204	23	-0.4267125555053255
2204	24	-0.3594389703485916
2204	25	-0.3061567204533162
2204	26	-0.3423946862207856
2204	27	-0.3158325905046482
2204	28	-0.43346442721026435
2204	29	-0.434485311084579
2204	30	-0.451653701563437
2204	31	-0.3626934082181066
2204	32	-0.454705228776465
2204	33	-0.345898792118156
2204	34	-0.44050812510511506
2204	35	-0.4723679698339005
2204	36	-0.4420311348478876
2204	37	-0.32593279978069845
2204	38	-0.3136682443861787
2204	39	-0.3816143442986579
2204	40	-0.3870354040441839
2204	41	-0.2965735457508494
2204	42	-0.3021803491940993
2204	43	-0.2394107282242537
2204	44	-0.4006713840011926
2204	45	-0.4274055999641355
2204	46	-0.3210335868171583
2204	47	-0.36950415143390103
2204	48	-0.4386597528089631
2204	49	-0.3993271941755694
2204	50	-0.3653954604352698
2204	51	-0.3722394981496997
2205	1	-0.24457910733212884
2205	2	-0.31481688645616046
2205	3	-0.2195959060567958
2205	4	-0.23831034936801387
2205	5	-0.35435607484250653
2205	6	-0.4918512582781247
2205	7	-0.35141863802417106
2205	8	-0.3329710193632103
2205	9	-0.3857869145694188
2205	10	-0.36000106910414686
2205	11	-0.3699995110586549
2205	12	-0.3439958580113647
2205	13	-0.29307122773191097
2205	14	-0.3273718531120906
2205	15	-0.39424322654902966
2205	16	-0.46863793415519694
2205	17	-0.5183837099424977
2205	18	-0.47146343722895523
2205	19	-0.498518872696188
2205	20	-0.532080032572722
2205	21	-0.4495122258678421
2205	22	-0.5397478058618503
2205	23	-0.4986807733911576
2205	24	-0.41232698441160665
2205	25	-0.4442780802849704
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1205	27	-0.07259427191414858
1205	28	-0.1282186082740182
1205	29	-0.03291665195928317
1205	30	-0.019180691638247538
1205	31	0.02356181796133683
1205	32	-0.3853557165425272
1205	33	-0.04053117790022043
1205	34	-0.02776555017016591
1205	35	0.06243350946594717
1205	36	0.1272415341787223
1205	37	0.018311708093449397
1205	38	-0.06787374732136442
1205	39	-0.013568634529441681
1205	40	-0.032682048047309564
1205	41	-0.002441546268158845
1205	42	-0.030142895221899835
1205	43	-0.047353511365027856
1205	44	-0.006685282445403118
1205	45	-0.07619079619636793
1205	46	-0.05491642520491524
1205	47	-0.15706457431353016
1205	48	-0.15323787879304263
1205	49	-0.03625249472545988
1205	50	0.11105470069508172
1205	51	0.13932055677997113
1210	1	0.020784184273846762
1210	2	0.062472701678366604
1210	3	0.018779746923765828
1210	4	0.07220402901546663
1210	5	0.0026314994293024085
1210	6	-0.027907035218035503
1210	7	-0.09443835716119509
1210	8	-0.007193157051805343
1210	9	0.008396568052365438
1210	10	-0.03947779800552098
1210	11	0.04845946315914418
1210	12	-0.022952633600088035
1210	13	0.016040242011523986
1210	14	-0.03980787788012918
1210	15	0.05862299970061713
1210	16	-0.009704711986763925
1210	17	0.07653798005949852
1210	18	0.03786366476840897
1210	19	0.10267693650155962
1210	20	-0.07548201213734984
1210	21	0.059452708328208814
1210	22	0.06697681241996634
1210	23	0.14472826123560623
1210	24	0.020111738441002558
1210	25	0.08642335735078177
1210	26	0.05341672740898673
1210	27	0.05419138415255276
1210	28	0.005278338247300951
1210	29	-0.02805423947232555
1210	30	-0.011059040662296127
1210	31	0.007633845971511732
1210	32	0.08582306966486186
1210	33	0.03579722406563235
1210	34	-0.09093096881854734
1210	35	-0.012960585886766296
1210	36	0.021004071495134724
1210	37	0.08190755273212602
1210	38	-0.06896814635234705
1210	39	0.09020705199507972
1210	40	0.08863113921827193
1210	41	0.08118637527045536
1210	42	0.1866208965464935
1210	43	0.1252196024396035
1210	44	0.04846991049714333
1210	45	-0.507294968916824
1210	46	-0.16376537571938599
1210	47	0.03086572833139911
1210	48	0.0683215018766532
1210	49	0.06255184068457709
1210	50	0.09452563219850063
1210	51	0.13057835033227505
1211	1	-1.2433380556490938
1211	2	-1.1636405911491359
1211	3	-1.1720399095176468
1211	4	-1.1808084376413177
1211	5	-1.2794080863738984
1211	6	-1.2697472100846632
1211	7	-1.4115624124368331
1211	8	-1.299873241274291
1211	9	-1.3097594287862009
1211	10	-1.4779804098905238
1211	11	-1.4198058253213937
1211	12	-1.5438677812981716
1211	13	-1.3583847419343371
1211	14	-1.8121354811205919
1211	15	-1.8511519644863057
1211	16	-1.6867556283076475
1211	17	-1.8187581570675526
1211	18	-1.6023298802325776
1211	19	-1.4103083924848043
1211	20	-2.0415159295943752
1211	21	-1.9851060189490077
1211	22	-1.7349521541874253
1211	23	-1.46437582516036
1211	24	-1.6804747007508567
1211	25	-1.6292578321374585
1211	26	-1.5529512087696347
1211	27	-1.5505433520655885
1211	28	-1.660644280800316
1211	29	-1.8463290986898748
1211	30	-2.0498274720493725
1211	31	-1.732563866931077
1211	32	-1.6719315428129065
1211	33	-1.948657274798233
1211	34	-1.6332737617448494
1211	35	-1.9380085939385374
1211	36	-1.7565418989158523
1211	37	-1.5998137288821823
1211	38	-2.163515450491847
1211	39	-1.8105544390639992
1211	40	-2.042650814694003
1211	41	-1.8632060990429267
1211	42	-1.8824481794760324
1211	43	-2.145279526932061
1211	44	-2.0854293312535006
1211	45	-2.3090024192724847
1211	46	-2.12905833289188
1211	47	-1.866860141062002
1211	48	-1.5985319485284073
1211	49	-1.803575159908192
1211	50	-1.6502475104610532
1211	51	-1.7180434635385033
1208	1	0.005378803020605005
1208	2	-0.04963857640881386
1208	3	0.05187340068347979
1208	4	0.14305787630229005
1208	5	0.03487386103621404
1208	6	0.11262137259431171
1208	7	8.282046706682422E-4
1208	8	-0.02817772392800677
1208	9	0.029793195542218598
1208	10	0.09087847075189615
1208	11	0.101764093629793
1208	12	0.08050175135095472
1208	13	0.012310508501904849
1208	14	-0.86622236177449
1208	15	-0.412275548141082
1208	16	-0.22288154383779357
1208	17	-0.10961921460532409
1208	18	-0.028140762987320045
1208	19	0.07091233351051329
1208	20	0.058910301348824134
1208	21	0.12371934008805141
1208	22	0.14187718410526884
1208	23	-0.37044426175631173
1208	24	-0.09060368574649402
1208	25	-0.3569424305669955
1208	26	-0.18940179310173022
1208	27	0.006352111037685404
1208	28	0.016960388116430636
1208	29	0.08403242091797125
1208	30	0.1305258321821583
1208	31	0.13892798800762307
1208	32	0.0402664972637794
1208	33	0.06268737064191043
1208	34	0.028071206570345453
1208	35	0.12662937702853583
1208	36	0.06509535742110728
1208	37	0.12183299845629136
1208	38	0.23082551847770816
1208	39	0.2816443615628117
1208	40	0.060502562435537754
1208	41	0.047472096415585696
1208	42	0.09486959701981235
1208	43	0.0803409312922092
1208	44	0.04490808035048488
1208	45	0.16545920451637386
1208	46	0.11118743242244378
1208	47	0.18633029494846554
1208	48	0.21733405976015518
1208	49	0.20175127394403702
1208	50	0.15806444136885744
1208	51	0.2912036779410414
1209	1	-0.06884242373018878
1209	2	0.02435651264445582
1209	3	0.05753300491170066
1209	4	-0.45066325956210207
1209	5	-0.21024091770930653
1209	6	-0.020404282018866127
1209	7	-0.03431618147639526
1209	8	0.10130983079639222
1209	9	0.04080739538755651
1209	10	0.03834119962388982
1209	11	0.1160249406910836
1209	12	0.08392585812245201
1209	13	0.0376102025690237
1209	14	0.18739691969916095
1209	15	0.11458153837969576
1209	16	0.0819896593441527
1209	17	0.25609658514882483
1209	18	0.13970516761244056
1209	19	0.023341465221854207
1209	20	0.007800716992377943
1209	21	-0.02820082775911459
1209	22	0.12064303916147878
1209	23	0.1832122985475877
1209	24	0.06998796934114182
1209	25	0.08691226209831626
1209	26	0.2485782488600634
1209	27	0.23578451774048403
1209	28	0.1728623047412512
1209	29	0.13218126512018813
1209	30	0.1486710717371551
1209	31	0.19696527299965538
1209	32	0.13799187243977684
1209	33	0.1492106113738103
1209	34	0.27884374100705145
1209	35	0.08220287023978301
1209	36	0.11980835664164147
1209	37	0.22073589172484986
1209	38	0.18421588944828216
1209	39	0.18417531685594213
1209	40	0.13804561024178952
1209	41	0.057188007081954595
1209	42	0.08956491190269134
1209	43	0.027286069793849066
1209	44	0.08042833569506769
1209	45	0.09994581956777893
1209	46	0.0947354527933193
1209	47	0.13686960562900552
1209	48	0.0932922951466999
1209	49	0.08079779854036317
1209	50	0.02458466282359595
1209	51	0.06941928513924367
1214	1	0.10531035203813843
1214	2	0.07195856923282662
1214	3	0.12991887425696902
1214	4	0.18117503845420657
1214	5	0.24263464224907594
1214	6	0.23167843375813746
1214	7	0.25282315343497785
1214	8	0.27900831075309185
1214	9	0.28608792078788525
1214	10	0.17198057918133003
1214	11	0.08063302995866195
1214	12	0.11298370193659579
1214	13	0.12208965977564645
1214	14	0.2564220216374409
1214	15	0.2191738876785223
1214	16	0.3394478185572396
1214	17	0.2930162764050408
1214	18	0.23108010441738003
1214	19	0.2150052774191593
1214	20	0.2170916953536306
1214	21	0.27056907498241145
1214	22	0.29528788523889915
1214	23	0.22108426076823662
1214	24	0.16958677572446135
1214	25	0.18777257858360485
1214	26	0.29909521889752
1214	27	0.2233079943977998
1214	28	0.19202892150376982
1214	29	0.30127957804135974
1214	30	0.2660520385803622
1214	31	0.27485145914998554
1214	32	0.24436260551961198
1214	33	0.2897608878760707
1214	34	0.23565036743967482
1214	35	0.18221366238233117
1214	36	0.16277436003645818
1214	37	0.09754461948470095
1214	38	0.2839887119925564
1214	39	0.24013186220831528
1214	40	0.2176931568530307
1214	41	0.22943235089648795
1214	42	0.3606515771613381
1214	43	0.347588509596072
1214	44	0.3095682554663419
1214	45	0.35564383172221525
1214	46	0.1517266020230288
1214	47	0.1551094685630865
1214	48	0.25949949871627354
1214	49	0.3095082095960855
1214	50	0.22034952487795678
1214	51	0.42023579423372937
1215	1	0.5138643841988788
1215	2	0.6211254755677871
1215	3	0.5648926314736826
1215	4	0.7037036909238275
1215	5	0.7846452848096703
1215	6	0.7491602374589981
1215	7	0.8148464682328473
1215	8	0.7100423220816587
1215	9	0.7901981663890716
1215	10	0.8072003799386032
1215	11	0.6725797601792998
1215	12	0.6828714054715235
1215	13	0.6349546576447587
1215	14	0.7010257200353323
1215	15	0.7156223829505564
1215	16	0.8113410625454733
1215	17	0.7786826332327408
1215	18	0.7006403225253095
1215	19	0.6254883470553381
1215	20	0.7841153329700177
1215	21	0.766936185630712
1215	22	0.7393772213622682
1215	23	0.70053918850342
1215	24	0.6508134832232599
1215	25	0.7699364761927328
1215	26	0.7485451318510457
1215	27	0.6588029854139918
1215	28	0.7199409843083657
1215	29	0.8154286205587766
1215	30	0.8397820698214673
1215	31	0.8837254753229757
1215	32	0.7227577436866497
1215	33	0.6892923930306409
1215	34	0.7244050452660815
1215	35	0.7757396980893532
1215	36	0.6847053329914274
1215	37	0.7501335595648584
1215	38	0.8229989318112061
1215	39	0.7555867807814565
1215	40	0.8404968993718356
1215	41	0.976852129063623
1215	42	0.8913952092976807
1215	43	0.8361668898602446
1215	44	0.7713434652661668
1215	45	0.701486394491809
1215	46	0.7294602270716801
1215	47	0.8017284850216768
1215	48	0.8129705564973477
1215	49	0.9204675904373296
1215	50	0.7734571791984486
1215	51	0.7227311312598381
1212	1	0.7298590022107803
1212	2	0.7670841444993961
1212	3	0.6787228056872578
1212	4	0.8723482993513372
1212	5	0.8703059871547225
1212	6	1.100033833320861
1212	7	1.026810826617858
1212	8	0.9413640709176363
1212	9	0.9705520899599414
1212	10	0.9972724586695456
1212	11	0.9182647835815736
1212	12	0.9810889848245878
1212	13	0.9037631319038155
1212	14	0.9502545960825586
1212	15	0.9210284612604553
1212	16	0.9488707778766567
1212	17	1.0568895823024747
1212	18	1.0417072483446645
1212	19	0.9328914344119497
1212	20	0.8829181231573884
1212	21	0.9623183783766152
1212	22	0.9021320849186978
1212	23	0.8684039454107477
1212	24	0.9324374608144055
1212	25	1.1062716088329907
1212	26	1.001666750803821
1212	27	0.9537747240282712
1212	28	0.9970391605548059
1212	29	1.0756139041593968
1212	30	1.0304661758554943
1212	31	0.8959959040090482
1212	32	0.9028295526242331
1212	33	1.0211581614152365
1212	34	0.8069632655759378
1212	35	0.9367233434423667
1212	36	0.9358057677127931
1212	37	1.0475883176611234
1212	38	0.8371463716881848
1212	39	0.8342997021627525
1212	40	0.9905421588015173
1212	41	0.9572509332204788
1212	42	0.957158084725588
1212	43	0.9706282544948976
1212	44	0.7990582892925566
1212	45	1.0074347220526718
1212	46	0.9086912508440186
1212	47	0.982902764522926
1212	48	0.8794501546312858
1212	49	1.0174678989647994
1212	50	0.9147088391180382
1212	51	0.9528691528700755
1213	1	1.6201744123361088
1213	2	1.6340169928007775
1213	3	1.6534668432332538
1213	4	2.0071695610780864
1213	5	2.1045419764699673
1213	6	2.0637728762367153
1213	7	2.1171775384738147
1213	8	2.101078754314784
1213	9	2.211405909859213
1213	10	2.1467120405458253
1213	11	1.9657560936693095
1213	12	2.037448323330935
1213	13	2.043054886456858
1213	14	2.1837381671434954
1213	15	2.132808769956874
1213	16	2.1916074076437866
1213	17	2.2313331443932753
1213	18	2.2620867093614265
1213	19	2.2054013874431035
1213	20	2.257800939832677
1213	21	2.208880683052513
1213	22	2.164533237578844
1213	23	2.167488829316966
1213	24	2.243301177877484
1213	25	2.251919838133837
1213	26	2.2341118485249964
1213	27	2.233214473773913
1213	28	2.320778243388638
1213	29	2.3668049721695468
1213	30	2.3668680968654456
1213	31	2.2759823055219712
1213	32	2.3379967499683474
1213	33	2.3454883154597823
1213	34	2.3157426725180983
1213	35	2.2379016045532083
1213	36	2.256811680812852
1213	37	2.1738023721897193
1213	38	2.3608950918625453
1213	39	2.237049045806046
1213	40	2.2486549444124293
1213	41	2.382956571702664
1213	42	2.357263361265762
1213	43	2.2471188853711084
1213	44	2.2796920285454014
1213	45	2.3291080851384436
1213	46	2.3341268727142683
1213	47	2.358485500182546
1213	48	2.368011105446172
1213	49	2.3102848761380947
1213	50	2.3774067534357393
1213	51	1.9595556637618223
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96.0
3	86.0
4	168.0
5	31.0
6	1485.0
7	16202.0
8	67682.0
9	117076.0
10	115554.0
11	98595.0
12	82741.0
13	58335.0
14	38762.0
15	26913.0
16	20029.0
17	15192.0
18	12886.0
19	11322.0
20	10700.0
21	10399.0
22	10539.0
23	10734.0
24	11613.0
25	12155.0
26	12793.0
27	14325.0
28	16088.0
29	18629.0
30	21796.0
31	26191.0
32	33277.0
33	44199.0
34	62233.0
35	95388.0
36	166131.0
37	405656.0
38	1508713.0
39	66075.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.809613399898275	27.84687964485529	25.56796858411231	26.77553837113412
2	22.656028906134992	34.71803834297087	25.147973277626228	17.477959473267905
3	23.2207094999003	31.41950603061951	26.037817852263558	19.321966617216635
4	23.503066695022266	30.59151363028724	25.545379709346882	20.360039965343617
5	24.70243138213505	29.987941391520827	23.8646559315113	21.444971294832822
6	19.227092713132546	30.09722222650905	27.213704633202845	23.461980427155556
7	20.983445565479965	35.618468106040474	24.997785602496666	18.40030072598289
8	26.184716767109332	30.849135369965968	24.884770010725887	18.081377852198806
9	20.10776005099387	36.779175238956604	24.627971619996885	18.485093090052647
10	20.69642863755936	30.853782883976088	28.88114551224998	19.568642966214572
11	20.041317978073202	31.14319450805435	24.129230093116675	24.686257420755773
12	26.925842688306712	29.498797283842865	24.853479729124988	18.721880298725434
13	25.838864449035448	30.24726602500992	24.220484581905673	19.693384944048955
14	20.346369246134255	36.94683478358557	23.994869335328318	18.71192663495185
15	19.946950846508688	36.042128213026245	24.570295066626255	19.440625873838815
16	25.487291215471096	30.450465632284907	24.34186017728996	19.720382974954035
17	20.498792973969536	35.86961587835721	24.039464395895894	19.592126751777357
18	25.7425904517604	29.870236079727285	24.87635579657234	19.51081767193997
19	20.291847798410206	30.84750366121458	24.12457234390263	24.73607619647258
20	20.69648894411533	35.63818328058632	24.18112502677474	19.484202748523607
21	20.145519231641668	30.927928909386566	24.604854118143024	24.321697740828743
22	20.909370935513284	35.14861630751548	23.946080715960782	19.99593204101046
23	21.142182508449466	29.716036236245507	23.959164776147045	25.18261647915799
24	26.298874440491502	29.10570606083798	24.9409630582432	19.65445644042731
25	20.557320850353612	30.6756388083396	27.94291925442836	20.824121086878428
26	20.4701574352086	30.927925736804678	24.9445668062579	23.657350021728824
27	20.81328298215899	31.025289984247767	28.001411034462325	20.160015999130913
28	24.281810833308384	30.195349428655465	24.774621148405135	20.748218589631016
29	20.23048829456625	33.89987811694539	24.845840621162218	21.023792967326145
30	21.391416582410216	32.70199635320798	24.98006239298196	20.926524671399843
31	20.90364113587021	31.61824317547414	25.133697596081888	22.34441809257376
32	23.19947759197304	31.20363389591156	25.19637363407182	20.400514878043587
33	22.186660577437863	31.924510020227142	24.42926594449647	21.459563457838517
34	22.0058093242988	32.282208975196184	25.068944063342123	20.643037637162887
35	22.379393318061304	32.28104891235414	24.575392697140153	20.764165072444403
36	22.484969890403104	31.88617575561	24.865623212951522	20.76323114103537
37	22.690836366890988	30.836397753804228	25.29899124884843	21.17377463045635
38	23.149229411365333	31.129126830803795	24.600478956171806	21.121164801659063
39	22.067387796304075	31.54288433544348	25.348392888609634	21.041334979642816
40	23.188942515561045	31.12353998993304	24.539310058349482	21.148207436156433
41	23.300596339377996	31.610708863986293	24.802107479348166	20.286587317287548
42	23.765921023759905	30.38014178356203	25.020191087775757	20.833746104902307
43	22.812746308877273	30.750673848624622	25.319796490617346	21.11678335188076
44	23.74718168727823	30.181637284618567	25.2905647241371	20.7806163039661
45	23.26878380718037	30.448721410779594	25.674831980608907	20.607662801431122
46	22.758674538053903	30.53388633439487	25.55838900532334	21.149050122227884
47	22.7781163926458	31.459375216992125	24.59818073804325	21.164327652318825
48	24.76089047005946	29.818575489853583	25.01651053155888	20.404023508528073
49	23.010342392699837	31.249074147227873	24.79560256967351	20.944980890398785
50	22.733988133356995	30.903053075836954	25.57136574413419	20.79159304667186
51	22.957745653019902	31.87597153766889	24.47656165856004	20.68972115075117
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3201.0
1	3199.0
2	3197.0
3	3192.0
4	3187.0
5	2981.0
6	2775.0
7	2661.0
8	2547.0
9	2601.0
10	2655.0
11	2786.5
12	2918.0
13	3223.0
14	3528.0
15	4106.0
16	4684.0
17	5665.0
18	6646.0
19	8018.5
20	9391.0
21	11653.5
22	13916.0
23	17202.5
24	20489.0
25	27185.5
26	42210.5
27	50539.0
28	58546.0
29	66553.0
30	80999.5
31	95446.0
32	108809.5
33	122173.0
34	145144.0
35	168115.0
36	192091.5
37	216068.0
38	240793.0
39	265518.0
40	279143.5
41	292769.0
42	311947.5
43	331126.0
44	334809.0
45	338492.0
46	320755.0
47	303018.0
48	269745.0
49	236472.0
50	210727.5
51	184983.0
52	161414.5
53	137846.0
54	118813.0
55	99780.0
56	88310.5
57	76841.0
58	59583.5
59	42326.0
60	37653.0
61	32980.0
62	25296.5
63	17613.0
64	14973.5
65	12334.0
66	10647.5
67	8961.0
68	7404.0
69	5847.0
70	5216.5
71	4586.0
72	4016.0
73	3446.0
74	2901.5
75	1975.5
76	1594.0
77	1286.0
78	978.0
79	860.0
80	742.0
81	649.0
82	556.0
83	512.0
84	468.0
85	391.5
86	315.0
87	298.5
88	282.0
89	254.5
90	227.0
91	206.0
92	185.0
93	179.5
94	174.0
95	205.5
96	237.0
97	378.5
98	520.0
99	2913.0
100	5306.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01990256076529512
2	0.01567519514537972
3	0.03563946927738893
4	0.030301263056619857
5	0.02406821301849642
6	0.027524161554485654
7	0.019439710514939416
8	0.029745842756193016
9	0.03653431309474329
10	0.01410150429417034
11	0.01706374589644682
12	0.01922371373144009
13	0.01959399393172465
14	0.02045798106572196
15	0.023913929601711188
16	0.0242533531186387
17	0.031103536823903065
18	0.013669510727171687
19	0.02295737241764274
20	0.02453106326885212
21	0.016354042179234748
22	0.02576533060313399
23	0.028974425672266844
24	0.01373122409388578
25	0.016570038962734075
26	0.02632075090356083
27	0.018915146897869623
28	0.024685346685637356
29	0.02462363331892326
30	0.0210134013661488
31	0.0140089342440992
32	0.012651240176389146
33	0.0029005282355623893
34	0.01484206469473946
35	0.01363865404381464
36	0.01419407434424148
37	0.01987170408193807
38	0.012620383493032098
39	0.012651240176389146
40	0.014996348111524692
41	0.016045475345664282
42	0.015366628311809252
43	0.01379293746059987
44	0.01157125625889251
45	0.016600895646091123
46	0.016354042179234748
47	0.014317501077669667
48	0.01336094389360122
49	0.01644661222930589
50	0.01894600358122667
51	0.01345351394367236
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3240789.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.28704135659247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.10842124267258	47.368997325044134
2	2.1186840700879013	2.0884733876795214
3	0.4688954183593019	0.6933140362977491
4	0.19627957225723924	0.3869615758118732
5	0.12486434359163176	0.3077097033282264
6	0.054026856413773555	0.15976943438594043
7	0.0397469755084217	0.1371307577978134
8	0.040629982097449245	0.16020252863636741
9	0.03358125012057996	0.14896084171491905
>10	0.13841034776228778	1.2734392058552195
>50	0.1767213039691474	7.013047156961107
>100	0.4987824349541413	39.54445048323036
>500	6.390386019886536E-4	0.1787676189521984
>1k	1.9240878362925554E-4	0.16855695464288167
>5k	1.2475482007160682E-4	0.3702189896617105
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	6812	0.2101957270282021	Illumina Single End PCR Primer 1 (100% over 51bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC	5230	0.1613804539573542	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.2697799825906593	0.0	0.0
2	0.2737604947437183	0.0	0.0
3	0.2778027202634914	0.0	0.0
4	0.2850848975357544	0.0	0.0
5	0.2926139282748738	0.0	0.0
6	0.3710516173684865	0.0	0.0
7	3.2482522003129484	0.0	0.0
8	3.2635571152580436	0.0	0.0
9	3.27247469674823	0.0	0.0
10	3.329868127792337	0.0	0.0
11	3.4815595831755783	0.0	0.0
12	3.596223018530364	0.0	0.0
13	3.6920638770373513	0.0	0.0
14	3.7134784152871414	0.0	0.0
15	3.7656570668439073	0.0	0.0
16	3.7925332380478953	0.0	0.0
17	3.832153219478343	0.0	0.0
18	3.8568694228473377	0.0	0.0
19	3.8719274843255764	0.0	0.0
20	3.883683880684611	0.0	0.0
21	3.8978162416621385	0.0	0.0
22	3.914787417508514	0.0	0.0
23	3.9284877849190427	0.0	0.0
24	3.94366927313071	0.0	0.0
25	3.9815921369765204	0.0	0.0
26	4.0255629107603115	0.0	0.0
27	4.079438679901715	0.0	0.0
28	4.105882857538704	0.0	0.0
29	4.13100019779134	0.0	0.0
30	4.1548832707096945	0.0	0.0
31	4.232580399402738	0.0	0.0
32	4.290837817580842	0.0	0.0
33	4.338881673567764	0.0	0.0
34	4.374243432694939	0.0	0.0
35	4.405439539568913	0.0	0.0
36	4.419942180746726	0.0	0.0
37	4.432809417706614	0.0	0.0
38	4.447157775467641	0.0	0.0
39	4.462740400562949	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGTCT	5425	0.0	41.309395	21
ACGTCTG	4765	0.0	41.27095	22
CGTCTGA	4670	0.0	41.243244	23
CACACGT	8215	0.0	41.166187	19
ACACGTC	7145	0.0	41.15879	20
GCACACG	9915	0.0	41.12015	18
AGCACAC	10540	0.0	39.813847	17
AGAGCAC	11180	0.0	39.408836	15
GGGCGCC	1090	0.0	39.007744	44
GAGCACA	11610	0.0	37.696705	16
CTCGGTG	1290	0.0	37.49479	38
AGGGCGC	1000	0.0	36.668774	43
CACGTCA	1850	0.0	36.48705	21
CGTCTAG	715	0.0	36.18993	23
TCTCGGT	1355	0.0	35.366276	37
CACGTAG	1185	0.0	35.317	21
GTCTGAG	645	0.0	35.230927	24
ACACGTA	1785	0.0	35.16861	20
GTCTGAA	4975	0.0	34.95818	24
AAGAGCA	13565	0.0	34.86869	14
>>END_MODULE