FastQCFastQC Report
Fri 19 Dec 2014
lane1_Undetermined_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240789
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG68120.2101957270282021Illumina Single End PCR Primer 1 (100% over 51bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC52300.1613804539573542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT54250.041.30939521
ACGTCTG47650.041.2709522
CGTCTGA46700.041.24324423
CACACGT82150.041.16618719
ACACGTC71450.041.1587920
GCACACG99150.041.1201518
AGCACAC105400.039.81384717
AGAGCAC111800.039.40883615
GGGCGCC10900.039.00774444
GAGCACA116100.037.69670516
CTCGGTG12900.037.4947938
AGGGCGC10000.036.66877443
CACGTCA18500.036.4870521
CGTCTAG7150.036.1899323
TCTCGGT13550.035.36627637
CACGTAG11850.035.31721
GTCTGAG6450.035.23092724
ACACGTA17850.035.1686120
GTCTGAA49750.034.9581824
AAGAGCA135650.034.8686914
Produced by FastQC (version 0.11.2)