FastQCFastQC Report
Fri 19 Dec 2014
lane1_Undetermined_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240789
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC123450.3809257560427414TruSeq Adapter, Index 14 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTTCA56750.032.199221
TCACAGA24650.028.7068931
GTCACAG25950.027.0082530
CACAGAT28950.025.45575732
AGTTCAG134850.024.0474051
GTAGGGA3350.021.4825152
TGTAGGG3700.019.4627151
CAGTCAC47050.019.2922828
GCTTGAA9600.018.95472716
ATGCCGT9700.018.5480484
CCAGTCA50250.018.19818527
CTCCAGT57100.016.88805825
TCCAGTC56950.016.36222626
AGTCACA46700.016.06496229
GAACTCC68850.015.72679222
AACTCCA66700.015.5940523
GTTCAGA233700.015.4839771
ACTCCAG64650.015.32500624
ACAGATC48850.015.13199233
CTTGAAA13050.014.46192717
Produced by FastQC (version 0.11.2)