FastQCFastQC Report
Fri 19 Dec 2014
cdc9deg_rad27d_rnh201_ACAGTG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecdc9deg_rad27d_rnh201_ACAGTG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28725865
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1302390.45338582493512375TruSeq Adapter, Index 5 (100% over 50bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG453280.1577950742301407TruSeq Adapter, Index 5 (100% over 39bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA415640.14469190048759195TruSeq Adapter, Index 5 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC209600.032.7920745
CTCGTAT195050.031.97399343
TCGTATG223700.030.83578544
ATCTCGT205750.029.983241
GATCTCG211450.029.04676440
TCTCGTA220200.028.33232342
AGTGATC227000.026.8688337
GTGATCT232550.026.27595538
TGATCTC238250.025.7321739
CAGTGAT352750.019.19108436
GTAGGGA98400.018.136932
TGTAGGG99650.017.877641
GACGTGT231900.016.1122671
ACAGTGA522750.014.78364745
ACGTGTG331950.013.6917671
CACAGTG617450.012.47251344
TAGGGAA176800.012.3056871
CGTGTGC448350.012.1414471
CGGTCAC32700.011.69790928
TCCGGTC32550.011.61343926
Produced by FastQC (version 0.11.2)