FastQCFastQC Report
Fri 19 Dec 2014
cdc9deg_rad27d_TGACCA_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecdc9deg_rad27d_TGACCA_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6020252
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG63290.1051284896379753Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG60600.038.83968418
ACACGAG35000.037.99331320
AGCACAC74350.037.5287917
CACACGA35700.037.43991519
GAGCACA91850.037.21737316
AGAGCAC92750.036.97786315
CACGAGA37700.035.3922621
CACACGT29200.034.36913719
AAGAGCA123900.031.895114
CACGTAG20050.030.86123521
ACGAGAT44100.029.95033522
ACACGTA21900.028.66472820
CGAGATC50050.026.34484723
GCACACA17150.025.71630518
CACACAG17000.025.28139519
GGTCGCC11850.025.0592244
ACACGTC15400.024.83796120
ACGTAGA25950.024.27855322
GTCGCCG12800.024.0781245
GCACAAG23700.023.7360418
Produced by FastQC (version 0.11.2)