FastQCFastQC Report
Fri 19 Dec 2014
cdc9deg_rad27d_TGACCA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecdc9deg_rad27d_TGACCA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6020252
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC16050.018.64379345
CGTGTGC93350.018.1671091
ACGTGTG56700.016.4048461
TCGTATG18650.016.2859344
CTCGTAT16150.015.74213443
GTGTGCT108750.015.5636772
AATCTCG18250.013.93024640
TGTGCTC122650.013.8365373
GTGCTCT125150.013.5238334
ACTGACC61000.013.49922843
ATCTCGT19200.013.2412141
CTGACCA64550.012.9659544
TCTCGTA20600.012.77828942
CACTGAC66250.012.56521542
GACGTGT36100.012.4151241
TGACCAA70100.012.16406845
GTCACTG73550.011.16486440
GCTCTTC157250.010.9609026
TGCTCTT158500.010.8910095
AGTCACT75800.010.80377239
Produced by FastQC (version 0.11.2)