FastQCFastQC Report
Fri 19 Dec 2014
cdc9deg_rad27d_PNK_GGCTAC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecdc9deg_rad27d_PNK_GGCTAC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8032226
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG411700.5125602790558931Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC51900.037.84704644
GTCGCCG53900.036.48444745
CTCGGTG55800.036.12879638
GTGGTCG55250.035.8373242
TCTCGGT57150.035.3147337
ATCTCGG61900.033.1504436
GGTGGTC60850.032.6131841
TGGTCGC61200.031.98548743
TCGGTGG63500.031.8188939
GCACACG28200.030.71788618
CGGTGGT67900.029.32620640
CACACGT24600.028.71950519
ACACTCT38300.026.7911741
GATCTCG78350.026.1327335
AGCACAC33850.026.1224317
GAGCACA39650.024.00362616
AGAGCAC40450.023.75139215
CACTCTT45250.023.3703042
ACACGTC19300.022.26683220
TCCGATC17150.021.77828427
Produced by FastQC (version 0.11.2)