FastQCFastQC Report
Fri 19 Dec 2014
cdc9deg_rad27d_PNK_GGCTAC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecdc9deg_rad27d_PNK_GGCTAC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8032226
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC397750.4951927398457165TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG52500.035.5704840
CTCGTAT60450.031.3009243
ATCTCGT60650.031.0873841
TCTCGTA64650.029.3370542
CGTATGC67550.028.97685845
ACATCTC64700.028.93276839
GCTACAT68050.028.13664836
TCGTATG71400.027.41438144
GGCTACA77550.024.77690135
TACATCT81400.023.35625838
CTACATC86850.021.89060837
CGGCTAC88650.021.75001134
CACGGCT101700.019.15830232
ACGGCTA101550.019.14217233
TCACGGC110100.017.86011331
GTCACAG22900.017.68467730
GTCACGG110900.017.67041430
GAACTCC146200.017.34338822
CAGTCAC132700.017.05639328
AGTCACG115550.017.03719929
Produced by FastQC (version 0.11.2)