FastQCFastQC Report
Fri 19 Dec 2014
LK_AA_Dna2_rnh201_PNK_CGATGT_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLK_AA_Dna2_rnh201_PNK_CGATGT_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9431305
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG211020.2237442220350206Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC28900.034.41214844
GTCGCCG30750.032.3418245
GTGGTCG31850.031.78965242
ACACTCT58050.030.1190071
TGGTCGC36050.027.33733443
TAGATCT323500.026.53429845
GGTGGTC39750.025.86793741
CACTCTT68800.025.8359052
AGTGTAG330350.025.82716441
GCACACG17450.025.7878418
GTGTAGA331350.025.77638442
GATCGAG21250.025.7288788
GAGTGTA333600.025.5755540
GTAGATC336750.025.55707644
TGTAGAT341500.025.02370543
AGAGTGT344000.024.80233639
AAGAGTG350300.024.40740238
GTAGGGA357600.024.16121331
TGTAGGG358250.024.12993830
GTGTAGG361500.023.9441229
Produced by FastQC (version 0.11.2)