FastQCFastQC Report
Fri 19 Dec 2014
LK_AA_Dna2_rnh201_PNK_CGATGT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLK_AA_Dna2_rnh201_PNK_CGATGT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9431305
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC202930.21516640592155592TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATG314950.027.0668145
CGTATGC38700.026.85870445
CACCGAT322300.026.42163344
GTCACCG325850.026.1199742
CTCGTAT38650.025.96207643
TCACCGA327650.025.95587743
TATCTCG38450.025.51252640
AGTCACC334700.025.45002441
CAGTCAC340500.025.02311940
TCCAGTC344800.024.65233238
CCAGTCA345450.024.63199639
TCGTATG42250.024.6019444
CTCCAGT351350.024.28240237
ACTCCAG354300.024.10562336
GAACTCC358850.023.87470634
TGAACTC372000.023.15170933
CTGAACT373050.023.06881932
CGTCTGA375750.022.97490929
ACGTCTG376100.022.94754628
GTCTGAA379400.022.7716730
Produced by FastQC (version 0.11.2)