FastQCFastQC Report
Fri 19 Dec 2014
K_AA_Dna2_PNK_ATCACG_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK_AA_Dna2_PNK_ATCACG_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11198584
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG307570.27465079513624224Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC39950.036.3835744
TGGTCGC41550.034.65760443
GTGGTCG43500.033.20743642
GTCGCCG44950.032.3865145
GGTGGTC50200.028.86501341
ACACTCT71850.027.2149661
GCACACG25900.026.06161918
CGGTGGT56150.026.00642440
CACTCTT85250.023.4633052
CACACGT24700.020.76919719
TAGATCT260800.020.6023345
GTGTAGA262950.020.22850842
GTAGATC267750.020.09276244
GAGTGTA264600.020.04266440
AGTGTAG267400.019.908741
AGCACAC37350.019.87939817
CTCTTTC114150.019.7502984
CTCGGTG74250.019.66637438
AGAGTGT272550.019.49106239
AGAGCAC41900.019.43902415
Produced by FastQC (version 0.11.2)