FastQCFastQC Report
Fri 19 Dec 2014
K_AA_Dna2_PNK_ATCACG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK_AA_Dna2_PNK_ATCACG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11198584
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC268810.24003927639422987TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC51050.028.4263345
GATCTCG49150.025.81774140
TCTCGTA50700.025.4274942
CTCGTAT51800.024.93095843
TCGTATG59550.024.44439944
ATCTCGT52950.024.30483641
GTCACAT262400.020.32082642
ACATCAC262100.020.31833345
CACATCA265600.020.1098844
TCACATC267700.019.88489243
AGTCACA274800.019.4205141
CAGTCAC273600.019.40700740
CCAGTCA280000.018.91520739
TCCAGTC282600.018.7411838
CTCCAGT288950.018.41497237
ACTCCAG290550.018.30582236
GAACTCC295350.018.10710534
CACGATC72700.017.33070637
TGAACTC315200.017.10955433
AACTCCA313200.017.07536935
Produced by FastQC (version 0.11.2)