FastQCFastQC Report
Fri 19 Dec 2014
K_AA_ACTTGA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK_AA_ACTTGA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4410858
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2522995.7199528980529415Illumina Single End PCR Primer 1 (100% over 51bp)
CCAAGAACAAGTTTAACCGCTCATTACTCCAAACGGATTTTTTTGCCAGAT188920.42830669225805956No Hit
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT149400.3387096115993759Illumina Single End PCR Primer 1 (100% over 39bp)
ACAGTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT74670.16928679182145515Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC259350.044.4277244
TGGTCGC259950.044.31651743
GTGGTCG262500.044.26315742
GTCGCCG260500.044.2488645
GGTGGTC265900.043.714141
CGGTGGT270350.043.21094540
GCACACG163150.042.90357218
CACACGT143950.042.1868119
GAGCACA184850.042.0054916
AGAGCAC187150.041.92206615
AGCACAC171650.041.7490117
CACGTAG61100.041.39144521
ACACGTC84950.040.7624920
ACACGTA65100.040.3689420
CACGTCA60950.040.23817421
ACGTCAG62250.039.6508722
ACCGCTC29750.039.32755716
ACGTAGA65000.039.18488322
TTAACCG30350.038.99413
AAGAGCA210800.038.91585514
Produced by FastQC (version 0.11.2)