FastQCFastQC Report
Fri 19 Dec 2014
K_AA_ACTTGA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK_AA_ACTTGA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4410858
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC2329225.280650612647245TruSeq Adapter, Index 8 (100% over 50bp)
GGCAAAAAAATCCGTTTGGAGTAATGAGCGGTTAAACTTGTTCTTGGAGAT188850.4281479929755163No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC152400.34551100942265656TruSeq Adapter, Index 8 (98% over 50bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG147790.33505952810088196TruSeq Adapter, Index 8 (100% over 38bp)
GGGAAAGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG73850.16742774308309177TruSeq Adapter, Index 8 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC285650.040.6807745
CTCGTAT268050.040.6323943
TCTCGTA268400.040.3866142
TCGTATG289350.040.24610544
AATCTCG270450.039.9898840
ATCTCGT272250.039.7833341
GAGCGTC31050.036.66418516
GAGCGGT29750.036.376126
AGCGTCG27400.036.12893717
TGAGCGG30250.035.70046625
ATGAGCG31100.034.86941524
GCGGTTA30850.034.86066428
AGAGCGT38650.034.28616315
CGGTTAA31850.033.90741729
AGCGGTT31900.033.8542727
AAGAGCG41700.033.4509714
AATCCGT33500.033.244029
GCGTCGT22700.032.6079218
GGAGTAA33950.032.47202718
CGTTTGG34300.032.40305313
Produced by FastQC (version 0.11.2)