FastQCFastQC Report
Fri 19 Dec 2014
HK_AA_Dna2_rad27_PNK_TGACCA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK_AA_Dna2_rad27_PNK_TGACCA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10290230
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG574840.5586269694652112Illumina Single End PCR Primer 1 (100% over 51bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC162060.15748919120369514Illumina Single End PCR Primer 1 (100% over 40bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG131690.12797575953112805Illumina Single End PCR Primer 1 (100% over 41bp)
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT122890.11942395845379548Illumina Single End PCR Primer 1 (100% over 39bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC65850.041.3100444
GTCGCCG67500.040.2335745
GTGGTCG70700.038.4761842
GGTGGTC77200.035.3531941
TGGTCGC76850.035.27997643
ACACTCT92350.033.989731
GCACACG31950.033.2394718
CGGTGGT85950.032.0419240
CACTCTT99900.031.5992852
GATCGAG29100.030.1542348
GAGCACA47850.028.02510816
CTCGGTG189800.027.59763344
AGCACAC42500.027.52944217
AGAGCAC50100.027.3503315
TCTCGGT194950.026.8916743
TCGGTGG198000.026.5683445
CACACGT25750.026.2136219
ATCTCGG203050.025.88540342
TAGATCT699600.025.3882345
TCGAGAT53800.024.84179310
Produced by FastQC (version 0.11.2)