FastQCFastQC Report
Fri 19 Dec 2014
HK_AA_Dna2_rad27_PNK_TGACCA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK_AA_Dna2_rad27_PNK_TGACCA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10290230
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC512500.4980452331969257TruSeq Adapter, Index 4 (100% over 50bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA157000.15257190558422892TruSeq Adapter, Index 4 (100% over 39bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA127830.12422462860402537TruSeq Adapter, Index 4 (100% over 40bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC121080.1176650084594805TruSeq Adapter, Index 4 (100% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC78450.034.12805645
CTCGTAT71300.033.35362643
TCGTATG82350.032.56643344
ATCTCGT75550.031.3287541
AATCTCG79500.029.40425940
TCTCGTA81550.029.21659542
ACTGACC684250.025.54511545
CACTGAC701900.024.96045544
GTCACTG722450.024.27225942
TCACTGA726900.024.11128443
AGTCACT731550.023.95210841
CAATCTC192200.023.89170645
GACCAAT218300.023.61179242
ACCAATC193800.023.54354143
CAGTCAC746050.023.45340740
CCAATCT195850.023.35454244
CCAGTCA755500.023.16302539
TCCAGTC757050.023.10965538
CTCCAGT768500.022.81218337
ACTCCAG774200.022.69653936
Produced by FastQC (version 0.11.2)