FastQCFastQC Report
Fri 19 Dec 2014
HK_AA_Dna2_rad27_GCCAAT_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK_AA_Dna2_rad27_GCCAAT_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20317019
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5269802.5937860273694677Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC570700.043.8806144
GTCGCCG573450.043.6701845
GCACACG1235600.043.5429718
GTGGTCG579550.043.3852442
TGGTCGC575850.043.3670543
CACACGT1061500.043.34028619
GAGCACA1381550.043.1300116
AGCACAC1277000.042.93317
AGAGCAC1398800.042.84423415
CACGTAG448750.042.62336721
GGTGGTC593850.042.37840741
ACACGTC614300.042.37391320
ACACGTA471800.042.24837520
CGGTGGT607150.041.77228540
CACGTCA419400.040.90125721
ACGTCAG421300.040.85565622
AAGAGCA1544750.040.6766414
ACGTAGA472500.040.51425622
CGTCAGA438500.039.2274523
CACACGA192250.039.04288519
Produced by FastQC (version 0.11.2)