FastQCFastQC Report
Fri 19 Dec 2014
HK_AA_Dna2_rad27_GCCAAT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK_AA_Dna2_rad27_GCCAAT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20317019
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC5116012.518090867562805TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC366600.1804398568510469TruSeq Adapter, Index 14 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC620200.040.49548345
TATCTCG608800.040.31189740
CTCGTAT618500.039.98465743
TCGTATG631000.039.80227744
TCTCGTA624750.039.4153942
ATCTCGT639650.038.434541
ATATCTC751650.032.6895939
CAATATC798400.030.87691337
AATATCT812200.030.30519738
CCAATAT824850.030.25776336
GCCAATA928850.026.91357635
TCAGACG404150.025.932862
TTCAGAC410650.025.4400441
CAGACGT412750.025.3380643
CGCCAAT1043100.024.06688934
GACGTGT1117350.023.3683531
ACGCCAA1167800.021.58752833
CACGCCA1200400.020.97892632
TCACGCC1236750.020.37323831
AGACGTG526050.020.1374744
Produced by FastQC (version 0.11.2)