FastQCFastQC Report
Fri 19 Dec 2014
52_repeat_GATCAG_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename52_repeat_GATCAG_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16832310
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG687930.4086961326163789Illumina Single End PCR Primer 1 (100% over 51bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC200090.1188725730455297Illumina Single End PCR Primer 1 (100% over 40bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG177070.10519649412350414Illumina Single End PCR Primer 1 (100% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC86000.039.3960644
GTGGTCG91000.037.42810442
GTCGCCG91450.037.1220445
TGGTCGC92600.036.36807643
CTCGGTG105750.032.90896638
GGTGGTC104050.032.88598341
GCACACG73350.031.84242218
CGGTGGT112350.030.69668440
TCTCGGT115900.029.98991237
ACACTCT122900.029.9721931
TCGGTGG117400.029.93090439
AGAGCAC97150.027.7969815
CACTCTT134950.027.7640272
GAGCACA97900.027.42279616
AGCACAC91150.026.61246717
CACACGT62850.026.52905519
ATCTCGG136300.026.40929836
GGCGCCG22850.026.09071445
GGGCGCC23950.025.45599244
CCCTAAG40050.025.22347510
Produced by FastQC (version 0.11.2)