FastQCFastQC Report
Fri 19 Dec 2014
52_repeat_GATCAG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename52_repeat_GATCAG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16832310
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC616590.366313358059589TruSeq Adapter, Index 9 (100% over 50bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG197070.11707840456835694TruSeq Adapter, Index 9 (100% over 39bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA173900.10331321131799498TruSeq Adapter, Index 9 (100% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC107750.031.57116545
GATCTCG101350.030.25635540
ATCTCGT105700.028.96929741
TCTCGTA106850.028.84701342
CTCGTAT107800.028.82245843
TCGTATG120250.028.45772744
AGATCTC120000.025.61025839
CAGATCT130900.023.59815238
TCAGATC149500.020.67724237
ATCAGAT187900.018.16376736
GTAGGGA50450.017.2647192
TGTAGGG53800.016.075121
GATCAGA218600.015.71573435
CGATCAG244500.013.9497434
GTCACAG58650.013.88662430
CACAGAT94350.013.40141132
TCCGGTC18150.013.38880826
CGGTCAC18500.013.25697528
TCACAGA86050.013.22987631
ACAGATC104600.012.56130333
Produced by FastQC (version 0.11.2)