FastQCFastQC Report
Fri 19 Dec 2014
44_gal_ACTTGA_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename44_gal_ACTTGA_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12673498
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG359750.2838600676782369Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC45850.038.95969444
GTCGCCG50400.035.48713745
GTGGTCG50400.035.12809442
TGGTCGC50750.034.75284643
CTCGGTG56750.032.4667438
GGTGGTC58350.030.49766341
TCTCGGT63700.028.89004737
TCGGTGG65900.028.19802739
CGGTGGT65050.027.73690840
ATCTCGG68000.027.52646836
GCACACG34000.027.06744818
ACACTCT65150.023.5210231
GAGCACA48000.022.26948516
CACACGT31550.021.53825419
AGCACAC46350.020.7299517
AGAGCAC52250.020.6734415
GATCTCG92250.020.2416635
CCCTAAG20500.020.08440810
CACTCTT78250.020.0732122
TCCGATC22200.019.5606527
Produced by FastQC (version 0.11.2)