FastQCFastQC Report
Fri 19 Dec 2014
44_gal_ACTTGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename44_gal_ACTTGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12673498
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC325300.256677359321002TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC64600.028.07106245
CTCGTAT62450.026.80393443
ATCTCGT65700.025.37518141
TCGTATG73100.024.99165344
TCTCGTA67050.024.86427142
AATCTCG69700.023.9508340
GAATCTC81650.020.1148239
TGAATCT97950.017.02014538
TTGAATC105800.015.75730837
CTTGAAT127250.014.35641536
CACTTGA136100.013.48900134
ACTTGAA143700.012.91650235
ACACTTG148750.012.23613933
CACACTT152200.012.10659832
GTCACAC150500.012.04905930
GAACTCC208700.011.62206922
AGTCACA175400.011.48016429
GTAGGGA26500.011.4656892
TCACACT159050.011.45792431
CAGTCAC179100.011.31917328
Produced by FastQC (version 0.11.2)