FastQCFastQC Report
Fri 19 Dec 2014
43_gal_CAGATC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename43_gal_CAGATC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5821872
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC270140.46400882740122074TruSeq Adapter, Index 7 (100% over 50bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC60240.1034718729645722TruSeq Adapter, Index 7 (100% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG37600.035.0035940
CGTATGC41800.033.2099645
CTCGTAT41650.031.81704143
TCTCGTA41500.031.60675642
ATCTCGT43100.030.69442641
TCGTATG47000.029.43992644
GATCATC52050.025.32941637
TCATCTC54300.024.11391439
AGATCAT61400.022.60815236
ATCATCT67900.019.61553838
CAGATCA74500.018.66311835
CCAGATC92950.014.934434
GTAGGGA16500.013.6385682
TGTAGGG17150.012.86817551
ACCAGAT110000.012.74272433
CACCAGA111900.012.50636132
TCACCAG116200.012.15973731
AGTCACC117700.011.94742329
GTCACCA119650.011.90314430
CAGTCAC123550.011.76535928
Produced by FastQC (version 0.11.2)