FastQCFastQC Report
Fri 19 Dec 2014
42_gal_GCCAAT_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename42_gal_GCCAAT_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8471900
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG263670.31122888608222476Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC33650.038.17410344
GTGGTCG36550.035.267342
GTCGCCG37600.034.52278545
TGGTCGC38300.033.3030643
CTCGGTG41250.032.0130338
GGTGGTC42000.030.74614341
TCTCGGT44850.029.29398537
CGGTGGT47000.027.95394140
TCGGTGG47850.027.78599239
ATCTCGG51500.026.34151336
GCACACG21100.025.3806118
ACACTCT43000.025.222981
CACTCTT48000.022.877942
AGAGCAC30100.021.15820115
AGCACAC28350.020.63696117
CACACGT19850.020.40419819
GAGCACA30350.020.3903716
GATCTCG71900.018.83619135
CTTTCCC44250.017.4424026
CCCTAAG13850.017.38141610
Produced by FastQC (version 0.11.2)