FastQCFastQC Report
Fri 19 Dec 2014
42_gal_GCCAAT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename42_gal_GCCAAT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8471900
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC259480.3062831242106257TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG39550.031.8557240
CGTATGC45450.028.51328845
CTCGTAT46650.027.2493143
ATCTCGT47450.026.55265441
TCTCGTA49400.025.82333242
TCGTATG51650.025.2648344
ATATCTC62950.020.33585539
CCAATAT82850.015.80443336
CAATATC82800.015.59660237
GCCAATA85250.015.25384635
AATATCT86950.014.95569838
CGCCAAT94550.013.89623634
CACGCCA97200.013.286386532
GTAGGGA19900.012.8919352
TCACGCC102200.012.65838231
ACGCCAA103350.012.56092133
GTCACGC103900.012.42961330
TGTAGGG20500.012.4133361
AGTCACG112350.011.63494529
CAGTCAC126800.011.03802628
Produced by FastQC (version 0.11.2)