FastQCFastQC Report
Fri 19 Dec 2014
41_gal_TTAGGC_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename41_gal_TTAGGC_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2769
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG140.5055976886962802Illumina Single End PCR Primer 1 (100% over 51bp)
CTCAAATCCGGCGTCAACCATACCAGCAGAGGAAGCATCAGCACCAGCACG40.1444564824846515No Hit
AATGGCCGTCTTCATTTCCATGCGGTGCACTTTATGCGGACACTTCCTACA30.10834236186348861No Hit
GGTCGTCAGCCAACGTGAGAGTGTCAAAAACGATAAACCAACCATCAGCAT30.10834236186348861No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)