FastQCFastQC Report
Fri 19 Dec 2014
3_AA_PNK_ACAGTG_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3_AA_PNK_ACAGTG_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3778753
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG58700.15534225179576436Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC9000.033.50013744
GTGGTCG10450.028.4211742
GTCGCCG10950.027.32887845
ACACTCT17300.026.5340081
TGGTCGC11650.025.68680243
GCACACG7100.025.03498518
GGTGGTC12850.023.4631341
TAGATCT77150.022.7771545
CACACGT5850.022.3074919
AGTGTAG79150.022.1731841
GTGTAGA79200.022.13077242
GTAGATC79950.022.00759544
GAGTGTA79950.021.97916240
CGGTGGT14000.021.53551940
AGAGTGT81650.021.4664339
TGTAGAT83350.020.94789743
CACTCTT22450.020.5460132
AAGAGTG85750.020.54500238
AGAGCAC11500.020.3476415
GTAGGGA88100.020.09940331
Produced by FastQC (version 0.11.2)