FastQCFastQC Report
Fri 19 Dec 2014
3_AA_PNK_ACAGTG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3_AA_PNK_ACAGTG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3778753
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC52680.13941107026577287TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC12550.024.02238745
ACACAGT73700.023.75019545
CACACAG74500.023.4347644
GTCACAC75350.023.2002642
TCACACA80400.021.79899643
CAGTCAC80400.021.771340
AGTCACA81150.021.57008641
TCGTATG14350.021.47947944
GATCTCG12650.021.34280640
ATCTCGT13250.021.22535341
CCAGTCA83100.021.14515339
TCCAGTC83700.020.99357438
CTCCAGT86100.020.30386237
CTCGTAT13900.020.23253343
GAACTCC87950.020.08116334
ACTCCAG87500.020.05613936
ACGTCTG92800.019.08040428
TGAACTC93200.019.02265433
CGTCTGA92800.019.00767129
CTGAACT95050.018.62873832
Produced by FastQC (version 0.11.2)