FastQCFastQC Report
Fri 19 Dec 2014
3_AA_CAGATC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3_AA_CAGATC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9292530
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5456585.872006870034318Illumina Single End PCR Primer 1 (100% over 51bp)
CCAAGAACAAGTTTAACCGCTCATTACTCCAAACGGATTTTTTTGCCAGAT323710.34835507660454146No Hit
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT306300.3296195976768437Illumina Single End PCR Primer 1 (100% over 39bp)
ACAGTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT170620.18360984575782915Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG569350.044.37190645
GGTCGCC571700.044.17376744
GTGGTCG576850.043.93541742
TGGTCGC575050.043.85774243
GGTGGTC583400.043.47299241
CGGTGGT593450.042.9491140
GCACACG267150.042.2454918
CACACGT238450.041.85784519
GAGCACA301100.041.3078816
AGCACAC280550.041.1098917
AGAGCAC304700.041.04874415
CACGTAG93250.040.07776321
ACACGTC149400.039.89459620
ACACGTA100700.038.92255820
ACGTCAG99800.038.39430222
CACGTCA98700.038.09271621
ACCGCTC50200.037.6488116
CTCGGTG691000.037.13620838
AAGAGCA358500.037.07263614
TCTCGGT694850.036.9466437
Produced by FastQC (version 0.11.2)