FastQCFastQC Report
Fri 19 Dec 2014
3_AA_CAGATC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3_AA_CAGATC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9292530
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC5156935.549543558105274TruSeq Adapter, Index 7 (100% over 50bp)
GGCAAAAAAATCCGTTTGGAGTAATGAGCGGTTAAACTTGTTCTTGGAGAT322880.3474618860525605No Hit
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT302880.32593922214940385TruSeq Adapter, Index 7 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCGTATGC171860.18494425091982483TruSeq Adapter, Index 7 (98% over 50bp)
GGGAAAGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT168600.18143605670361032TruSeq Adapter, Index 7 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC620450.040.80529445
CATCTCG604150.040.16382240
CTCGTAT612700.039.99583443
TCGTATG635100.039.88882444
TCTCGTA612400.039.7986742
ATCTCGT616750.039.46732341
GAGCGGT50400.034.4174626
TGAGCGG51500.033.90076425
TCATCTC718000.033.7795639
GATCATC720600.033.6951537
ATGAGCG53000.032.98375724
ATCATCT737700.032.9140938
GAGCGTC49700.032.8651216
AGATCAT764000.032.83531636
GCGGTTA54150.032.15862728
AGCGTCG44950.032.0836217
CGGTTAA54200.032.0044329
AATCCGT55950.031.4058579
CAGATCA805150.031.380735
GACTGTA42350.031.1849027
Produced by FastQC (version 0.11.2)