FastQCFastQC Report
Wed 22 Oct 2014
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4641352
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATATCTCGTATGCCG83610.1801414760181947TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCATATCTCGTATGCCG66220.14267394500567937TruSeq Adapter, Index 6 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA75000.042.2202452
ACACGTC64200.042.2163313
GATCGGA75250.042.1761971
TCGGAAG75600.041.4689753
CGGAAGA76400.040.917344
CACACGT67500.040.8469112
CACGTCT65950.040.6883114
GCACACG68900.040.52916311
CGTCTGA64600.040.4896816
ACGTCTG65450.040.32005715
AGAGCAC79100.037.558818
CGCAATA10300.037.47948533
AGCACAC76500.037.352610
GAACTCC70550.034.4814421
GAGCACA85550.034.082879
CTGAACT75050.034.01236319
TGAACTC75300.033.45146620
GAAGAGC91500.033.3996966
AAGAGCA95500.031.95387
TCTGAAC81600.031.7852518
Produced by FastQC (version 0.11.2)