FastQCFastQC Report
Wed 22 Oct 2014
lane1_Undetermined_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4365636
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA46800.040.9668122
GATCGGA47100.040.9070931
ACACGTC38550.039.8604613
TCGGAAG47750.039.7291153
CGGAAGA47850.039.224324
CACGTCT39850.038.228514
ACGTCTG39050.038.14985315
GCACACG41750.038.07649211
CGTCTGA38850.038.0579316
CACACGT41100.037.98731212
AGAGCAC52600.033.63098
AGCACAC51300.032.42901610
GAACTCC45100.030.67120221
CTGAACT48150.030.2519619
GAAGAGC60750.029.8582556
CGCAATA4600.029.43473433
GAGCACA58750.029.270819
TGAACTC50200.028.40789620
GAATTCG2900.027.95252622
TTCGGTA2650.027.19509725
Produced by FastQC (version 0.11.2)