FastQCFastQC Report
Wed 22 Oct 2014
SV9_GCCAAT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV9_GCCAAT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17811124
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC8685594.87649740690144TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA927200.044.415881
ATCGGAA928800.044.3522722
ACACGTC922800.044.061313
CGTCTGA919950.044.05105616
TCGGAAG934650.044.0417633
ACGTCTG921450.044.02573815
CGGAAGA937000.043.900084
CACGTCT928350.043.75425714
CGCCAAT917200.043.6146533
CACACGT935450.043.5472312
CTCGTAT918800.043.49339742
TATCTCG919150.043.4931139
GTCACGC920950.043.48127729
ACGCCAA920900.043.46629332
TCTCGTA919850.043.43837441
TCGTATG922900.043.4269543
GCACACG940200.043.3846611
CGTATGC924000.043.3482244
AGTCACG924050.043.3435628
TCACGCC925050.043.2959830
Produced by FastQC (version 0.11.2)