FastQCFastQC Report
Wed 22 Oct 2014
SV8_ACAGTG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV8_ACAGTG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29913207
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1425290.47647515694321907TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT165600.040.69322242
TCTCGTA168100.040.0611941
TCGTATG170200.039.725643
CGTATGC171050.039.54035644
GATCTCG172300.039.020639
ACACGTC179000.038.5620713
ATCTCGT175400.038.42075340
CGTCTGA178950.038.3339616
GATCGGA187300.037.4559751
ATCGGAA187600.037.4484252
ACGTCTG185400.037.13382715
CACGTCT192100.036.0377914
TCGGAAG202200.034.8767553
GTATGCC197500.034.42722745
CGGAAGA206800.034.1009674
GCACACG211150.032.81841711
CACACGT213350.032.5116412
AGTGATC216250.031.1314836
GTGATCT224650.030.09768537
TGATCTC243250.027.8702338
Produced by FastQC (version 0.11.2)